Journal of Cellular Biochemistry

MicroRNA-212 inhibits proliferation of gastric cancer by directly repressing retinoblastoma binding protein 2

Authors

  • Zeng Jiping,

    1. Department of Microbiology/Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, Shandong University School of Medicine, Jinan, China
    2. Department of Biochemistry, Shandong University School of Medicine, Jinan, China
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  • Fang Ming,

    1. Department of Microbiology/Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, Shandong University School of Medicine, Jinan, China
    2. Institute of Bacterial Infectious Diseases, Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan, China
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  • Wang Lixiang,

    1. Department of Microbiology/Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, Shandong University School of Medicine, Jinan, China
    2. Department of Pharmacology, Shandong University School of Medicine, Jinan, China
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  • Liang Xiuming,

    1. Department of Microbiology/Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, Shandong University School of Medicine, Jinan, China
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  • Shen Yuqun,

    1. Department of Microbiology/Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, Shandong University School of Medicine, Jinan, China
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  • Yu Han,

    1. Department of Microbiology/Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, Shandong University School of Medicine, Jinan, China
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  • Liu Zhifang,

    1. Department of Microbiology/Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, Shandong University School of Medicine, Jinan, China
    2. Department of Biochemistry, Shandong University School of Medicine, Jinan, China
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  • Sun Yundong,

    1. Department of Microbiology/Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, Shandong University School of Medicine, Jinan, China
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  • Liu Shili,

    1. Department of Microbiology/Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, Shandong University School of Medicine, Jinan, China
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  • Chen Chunyan,

    1. Department of Hematology, Qilu Hospital, Shandong University School of Medicine, Jinan, China
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  • Jia Jihui

    Corresponding author
    1. Department of Microbiology/Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, Shandong University School of Medicine, Jinan, China
    • Correspondence to: Jia Jihui, Department of Microbiology/Key Laboratory for Experimental Teratology of the Chinese Ministry of Education, Shandong University School of Medicine, Jinan 250012, PR China.

      E-mail: jiajihui@sdu.edu.cn.

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  • ZJ, FM, and JJ designed the study; ZJ, FM, WL, SY, and LX performed the study; ZJ, FM, WL, LX, YH, LZ, and SY analyzed and interpreted the data; CC and JJ supervised the study; and ZJ, FM and JJ wrote the paper.
  • Conflicts of interest: No conflicts of interest were declared.
  • This manuscript has not been published in whole or in part nor is it being considered for publication elsewhere.
  • Zeng Jiping and Fang Ming contributed equally to this work.

ABSTRACT

Retinoblastoma binding protein 2 (RBP2), a newly found histone demethylase, is overexpressed in gastric cancer. We examined the upstream regulatory mechanism of RBP2 at the microRNA (miRNA) level and the role in gastric carcinogenesis. We used bioinformatics to predict that microRNA-212 (miR-212) might be a direct upstream regulator of RBP2 and verified the regulation in gastric epithelial-derived cell lines. Overexpression of miR-212 significantly inhibited the expression levels of RBP2, whereas knockdown of miR-212 promoted RBP2 expression. Furthermore, we identified the putative miR-212 targeting sequence in the RBP2 3′ UTR by luciferase assay. MiR-212 inhibited the colony formation ability of cells by repressing RBP2 expression and increasing that of P21CIP1 and P27kip1, both critical in cell cycle arrest. In addition, the expression of RBP2 and miR-212 in tumor tissue and matched normal tissue from 18 patients further supported the results in vivo. MiR-212 directly regulates the expression of RBP2 and inhibits cell growth in gastric cancer, which may provide new clues to treatment. J. Cell. Biochem. 114: 2666–2672, 2013. © 2013 Wiley Periodicals, Inc.

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