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Fig. S1. Conserved residues of Brr2p chosen for mutagenesis. An alignment of the amino acid sequence of regions of Brr2p from Homo sapiens (hsa), Drosophila melanogaster (dme), Caenorhabditis elegans (cel) and Saccharomyces cerevisiae (sce). The figure illustrates the conserved nature of amino acids R295, P841, G873 and F1149 that were mutated to create novel Brr2p mutants.

TABLE S1. Primers Used for Primer Extension (RT) and Oligomutagenesis

TABLE S2. Viability of U5 snRNA Mutants

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