The U5 snRNA internal loop 1 is a platform for Brr2, Snu114 and Prp8 protein binding during U5 snRNP assembly
Article first published online: 15 OCT 2013
© 2013 The Authors. Journal of Cellular Biochemistry Published by Wiley Periodicals Inc.
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Journal of Cellular Biochemistry
Volume 114, Issue 12, pages 2770–2784, December 2013
How to Cite
Nancollis, V., Ruckshanthi, J. P.D., Frazer, L. N. and O'Keefe, R. T. (2013), The U5 snRNA internal loop 1 is a platform for Brr2, Snu114 and Prp8 protein binding during U5 snRNP assembly. J. Cell. Biochem., 114: 2770–2784. doi: 10.1002/jcb.24625
- Issue published online: 15 OCT 2013
- Article first published online: 15 OCT 2013
- Accepted manuscript online: 16 JUL 2013 02:46AM EST
- Manuscript Accepted: 26 JUN 2013
- Manuscript Received: 28 AUG 2012
- Wellcome Trust. Grant Number: 079536
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Fig. S1. Conserved residues of Brr2p chosen for mutagenesis. An alignment of the amino acid sequence of regions of Brr2p from Homo sapiens (hsa), Drosophila melanogaster (dme), Caenorhabditis elegans (cel) and Saccharomyces cerevisiae (sce). The figure illustrates the conserved nature of amino acids R295, P841, G873 and F1149 that were mutated to create novel Brr2p mutants.
TABLE S1. Primers Used for Primer Extension (RT) and Oligomutagenesis
TABLE S2. Viability of U5 snRNA Mutants
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