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Saccharomyces boulardii and Bacillus subtilis B10 Modulate TLRs Mediated Signaling to Induce Immunity by Chicken BMDCs

Authors

  • Imran Rashid Rajput,

    1. Key Laboratory of Molecular Feed Sciences, Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Zhejiang, China
    2. Lasbela University of Agriculture, Water and Marine Sciences, Uthal, Balochistan, Pakistan
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  • Altaf Hussain,

    1. Key Laboratory of Molecular Feed Sciences, Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Zhejiang, China
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  • Ya Li Li,

    1. Key Laboratory of Molecular Feed Sciences, Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Zhejiang, China
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  • Xiaoping Zhang,

    1. Key Laboratory of Molecular Feed Sciences, Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Zhejiang, China
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  • Xin Xu,

    1. Key Laboratory of Molecular Feed Sciences, Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Zhejiang, China
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  • Mao Yu Long,

    1. Key Laboratory of Molecular Feed Sciences, Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Zhejiang, China
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  • Dong Yu You,

    Corresponding author
    1. Key Laboratory of Molecular Feed Sciences, Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Zhejiang, China
    • Correspondence to: Prof. Dr. Wei Fen Li, Dong Yu You, and Imran Rashid Rajput, Key Laboratory of Molecular Feed Sciences, Institute of Animal Nutrition and Feed Sciences, College of Animal Science, Zhejiang University, Zhejiang 310058, China.

      E-mail: wfli@zju.edu.cn (W.F.L.), dyyu@zju.edu.cn (D.Y.Y.), imran_dt@yahoo.com (I.R.R)

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  • Wei Fen Li

    Corresponding author
    1. Key Laboratory of Molecular Feed Sciences, Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Zhejiang, China
    • Correspondence to: Prof. Dr. Wei Fen Li, Dong Yu You, and Imran Rashid Rajput, Key Laboratory of Molecular Feed Sciences, Institute of Animal Nutrition and Feed Sciences, College of Animal Science, Zhejiang University, Zhejiang 310058, China.

      E-mail: wfli@zju.edu.cn (W.F.L.), dyyu@zju.edu.cn (D.Y.Y.), imran_dt@yahoo.com (I.R.R)

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ABSTRACT

Dendritic cells (DCs) are professional antigen-presenting cells (APCs) that play a critical role to activate immune response. They may be targeted for immunomodulation by microbes, including probiotics. In this study, chicken bone marrow dendrite cells (chi-BMDCs) were stimulated with lipopolysachride (LPS), Saccharomyces boulardii (Sb), Bacillus subtilis B10 (Bs), co-culture of Sb + Bs and phosphate buffer saline (PBS) as a control group (Ctr) at 3, 6, and 12 h intervals. Results revealed that treatment groups modulated the phenotype and biological functions of chi-BMDCs. Scan electron microscopy showed attachment of probiotics on the surface of chi-BMDCs. Additionally transmission electron microscopy (TEM) revealed efficiently engulfing and degradation of probiotics. Gene expression levels of MHC-II, CD40, CD80 and CD86 up-regulated in stimulated groups. Furthermore, toll-like receptors TLR1, TLR2, TLR4, and chicken specific TLR15 expressions were improved and downstream associated factors MyD88, TRAF6, TAB1, and NFκ-B mRNA levels increased in all treatment groups as compared to control. Surprisingly, NFκ-B response was noted significant higher in LPS treatment among all groups. Moreover, IL-1β, IL-17, IL-4, TGF-β, and IL-10 production levels were found higher, and lower concentration of INF-γ and IL-8 were observed in Sb, Bs, and Sb + Bs treatment groups. In contrast, LPS groups showed prominent increase in IL-12, INF-γ, and IL-8 concentration levels as compared to control group. Altogether, these results emphasize a potentially important role of Saccharomyces boulardii and Bacillus subtilis B10 in modulating immunological functions of chi-BMDCs by targeting specific toll like receptors (TLRs) and associated factors. The role of probiotics on chi-BMDCs functionality in a non-mammalian species have been presented for the first time. J. Cell. Biochem. 115: 189–198, 2014. © 2013 Wiley Periodicals, Inc.

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