The authors declare that no competing interests exist.
Putting Molecules in Their Place
Article first published online: 13 DEC 2013
© 2013 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry
Volume 115, Issue 2, pages 209–216, February 2014
How to Cite
Cinquin, B. P., Do, M., McDermott, G., Walters, A. D., Myllys, M., Smith, E. A., Cohen-Fix, O., Le Gros, M. A. and Larabell, C. A. (2014), Putting Molecules in Their Place. J. Cell. Biochem., 115: 209–216. doi: 10.1002/jcb.24658
- Issue published online: 13 DEC 2013
- Article first published online: 13 DEC 2013
- Accepted manuscript online: 21 AUG 2013 08:25AM EST
- Manuscript Accepted: 14 AUG 2013
- Manuscript Received: 10 AUG 2013
- NIH-NIGMS. Grant Number: P41 GM103445
- US DOE-BER. Grant Number: DE-AC02-05CH11231
- NIH-NIDDK. Grant Number: Intramural
- CORRELATED IMAGING;
- SOFT X-RAY;
Each class of microscope is limited to imaging specific aspects of cell structure and/or molecular organization. However, imaging the specimen by complementary microscopes and correlating the data can overcome this limitation. Whilst not a new approach, the field of correlative imaging is currently benefitting from the emergence of new microscope techniques. Here we describe the correlation of cryogenic fluorescence tomography (CFT) with soft X-ray tomography (SXT). This amalgamation of techniques integrates 3D molecular localization data (CFT) with a high-resolution, 3D cell reconstruction of the cell (SXT). Cells are imaged in both modalities in a near-native, cryopreserved state. Here we describe the current state of the art in correlative CFT-SXT, and discuss the future outlook for this method. J. Cell. Biochem. 115: 209–216, 2014. © 2013 Wiley Periodicals, Inc.