This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: [10.1002/jcb.24658]
Putting molecules in their place†
© 2013 Wiley Periodicals, Inc.
- Accepted manuscript online: 21 AUG 2013 08:25AM EST
- Manuscript Accepted: 14 AUG 2013
- Manuscript Received: 10 AUG 2013
- NIH-NIGMS. Grant Number: P41 GM103445
- US DOE-BER. Grant Number: DE-AC02-05CH11231
- NIH-NIDDK. Grant Number: Intramural
- Cited By
- Correlated imaging;
- soft x-ray;
Each class of microscope is limited to imaging specific aspects of cell structure and/or molecular organization. However, imaging the specimen by complementary microscopies and correlating the data can overcome this limitation. Whilst not a new approach, the field of correlative imaging is currently benefitting from the emergence of new microscope techniques. Here we describe the correlation of cryogenic fluorescence tomography (CFT) with soft x-ray tomography (SXT). This amalgamation of techniques integrates 3-D molecular localization data (CFT) with a high-resolution, 3-D cell reconstruction of the cell (SXT). Cells are imaged in both modalities in a near-native, cryopreserved state. Here we describe the current state of the art in correlative CFT-SXT, and discuss the future outlook for this method. J. Cell. Biochem. © 2013 Wiley Periodicals, Inc.