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Journal of Cellular Biochemistry

Pyk2 and Src Mediate Signaling to CCL18-Induced Breast Cancer Metastasis

Authors

  • Hai-Yan Li,

    1. Breast Cancer Center, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, Guangzhou, People's Republic of China
    2. Department of Breast and Thyroid Surgery, The 6th Affriciated Hospital of Sun Yat-Sen University, Sun-Yat-Sen University, Guangzhou, People's Republic of China
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  • Xiu-Ying Cui,

    1. Breast Cancer Center, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, Guangzhou, People's Republic of China
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  • Wei Wu,

    1. Breast Cancer Center, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, Guangzhou, People's Republic of China
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  • Feng-Yan Yu,

    1. Breast Cancer Center, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, Guangzhou, People's Republic of China
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  • He-Rui Yao,

    1. Breast Cancer Center, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, Guangzhou, People's Republic of China
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  • Qiang Liu,

    1. Breast Cancer Center, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, Guangzhou, People's Republic of China
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  • Er-Wei Song,

    Corresponding author
    1. Breast Cancer Center, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, Guangzhou, People's Republic of China
    • Correspondence to: Jing-Qi Chen, Department of Medical Oncology, No. 2 Affiliated Hospital, Guangzhou Medical University, No. 250, Changgang East Road, Guangzhou 510260, People's Republic of China. E-mail: chenjingqi2002@163.com

      Correspondence to: Er-Wei Song, Breast Cancer Center, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, Guangzhou 510120, People's Republic of China. E-mail: songerwei02@yahoo.com.cn

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  • Jing-Qi Chen

    Corresponding author
    1. Breast Cancer Center, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, Guangzhou, People's Republic of China
    2. Department of Medical Oncology, No. 2 Affiliated Hospital, Guangzhou Medical College, Guangzhou, People's Republic of China
    • Correspondence to: Jing-Qi Chen, Department of Medical Oncology, No. 2 Affiliated Hospital, Guangzhou Medical University, No. 250, Changgang East Road, Guangzhou 510260, People's Republic of China. E-mail: chenjingqi2002@163.com

      Correspondence to: Er-Wei Song, Breast Cancer Center, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, Guangzhou 510120, People's Republic of China. E-mail: songerwei02@yahoo.com.cn

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  • Hai-Yan Li, Xiu-Ying Cui, and Wei Wu contributed equally to this work.

ABSTRACT

Pyk2 and Src phosphorylation is initiated by CCL18, which promotes breast cancer metastasis via its functional G protein-coupled receptor PITPNM3. However, the function of Pyk2 and Src in CCL18-induced breast cancer metastasis is poorly understood. Quantitative reverse-transcription polymerase chain reactions (qRT-PCRs), Western blot, boyden chamber assay, and adherence assay were performed to delineate the consequences of Pyk2/Src in CCL18-induced breast cancer cells. Co-immunoprecipitation and immunofluorescence were performed to analyze the interaction of proteins. Upon the binding of CCL18 to PITPNM3, Pyk2 translocates from the cytoplasm to the plasma membrane to form a stable complex with PITPNM3, subsequently activating Src kinase. Moreover, upon stimulation with CCL18, Pyk2 and Src become essential for integrin alpha5/beta1 clustering-dependent adherence, migration, and invasion. Pyk2 and Src are important in CCL18-induced breast cancer metastasis. J. Cell. Biochem. 115: 596–603, 2014. © 2013 Wiley Periodicals, Inc.

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