Journal of Cellular Biochemistry

Requirement of miR-144 in CsA Induced Proliferation and Invasion of Human Trophoblast Cells by Targeting Titin

Authors

  • Ying Liang,

    1. National Engineering Laboratory for Rice and By-Product Deep Processing, Central South University of Forestry and Technology, Hunan Province, People's Republic of China
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  • Qinlu Lin,

    1. National Engineering Laboratory for Rice and By-Product Deep Processing, Central South University of Forestry and Technology, Hunan Province, People's Republic of China
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  • Feijun Luo,

    1. National Engineering Laboratory for Rice and By-Product Deep Processing, Central South University of Forestry and Technology, Hunan Province, People's Republic of China
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  • Wei Wu,

    1. National Engineering Laboratory for Rice and By-Product Deep Processing, Central South University of Forestry and Technology, Hunan Province, People's Republic of China
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  • Tao Yang,

    1. National Engineering Laboratory for Rice and By-Product Deep Processing, Central South University of Forestry and Technology, Hunan Province, People's Republic of China
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  • Shumei Wan

    Corresponding author
    1. Department of Obstetrics and Gynecology, Guangzhou General Hospital of Guangzhou Military Command, Guangdong Province, People's Republic of China
    • Correspondence to: Shumei Wan, Department of Obstetrics and Gynecology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong Province 510010, People's Republic of China.

      E-mail: wan_shumei@163.com

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ABSTRACT

MicroRNAs (miRNAs) are endogenous 19–25 nucleotide noncoding single-stranded RNAs that regulate gene expression by blocking the translation or decreasing the stability of mRNAs. In this study, with the treatment of CsA (Cyclosporin A), we showed that miR144 expression levels were decreased while titin mRNA expression levels were increased in human trophoblast (HT) cells, and identified titin as a novel direct target of miR-144. Overexpression of miR-144 suppressed titin and its downstream signaling molecule such as p-ERK1/2 and MMP2/9 expression, and attenuated cell proliferation and invasion. Forced expression of titin can partly rescue the inhibitory effect of miR-144 in the cells. Taken together, these findings will shed light to the role and mechanism of miR-144 in regulating HT cells proliferation and invasion via miR-144/titin axis, and miR-144 may serve as a potential therapeutic target in HT in the future. J. Cell. Biochem. 115: 690–696, 2014. © 2013 Wiley Periodicals, Inc.

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