Ling Wang and Dandan Wei contributed equally to this work.
The Combinational Effect of Vincristine and Berberine on Growth Inhibition and Apoptosis Induction in Hepatoma Cells
Article first published online: 23 JAN 2014
© 2013 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry
Volume 115, Issue 4, pages 721–730, April 2014
How to Cite
Wang, L., Wei, D., Han, X., Zhang, W., Fan, C., Zhang, J., Mo, C., Yang, M., Li, J., Wang, Z., Zhou, Q. and Xiao, H. (2014), The Combinational Effect of Vincristine and Berberine on Growth Inhibition and Apoptosis Induction in Hepatoma Cells. J. Cell. Biochem., 115: 721–730. doi: 10.1002/jcb.24715
- Issue published online: 23 JAN 2014
- Article first published online: 23 JAN 2014
- Accepted manuscript online: 14 NOV 2013 07:42AM EST
- Manuscript Accepted: 6 NOV 2013
- Manuscript Received: 31 OCT 2013
- National Natural Science Foundation of China. Grant Number: 81273224
- Huaxi Hospital of Sichuan University
- HEPATIC CARCINOMA CELLS
The use of vincristine, a known antitumor agent, in hepatoma therapy is limited particularly because of its toxic effect. Meanwhile, berberine has drawn increasing attention to its antineoplastic effect in recent years. In view of the advantages of combinational drug treatment reported in anti-cancer chemotherapy, we evaluated the effects of co-treatment of vincristine and berberine on hepatic carcinoma cell lines in this study. We find that combinational usage of these two drugs can significantly induce cell growth inhibition and apoptosis even under a concentration of vincristine barely showing cytotoxicity in the same cells when used alone. The underlying mechanism about this combinational effect was addressed in this study by monitoring the signals related to mitochondrial function, apoptotic pathway and endoplasmic reticulum stress. Our results suggest a new value of berberine as a potential adjuvant agent in cancer chemotherapy and provide a hopeful approach for developing hepatoma therapy by utilizing the combinational effect of vincristine and berberine. J. Cell. Biochem. 115: 721–730, 2014. © 2013 Wiley Periodicals, Inc.