Shiying Shao and Mingbo Nie contributed equally to this work.
Protective Action of Liraglutide in Beta Cells Under Lipotoxic Stress Via PI3K/Akt/FoxO1 Pathway
Version of Record online: 15 APR 2014
© 2014 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry
Volume 115, Issue 6, pages 1166–1175, June 2014
How to Cite
Shao, S., Nie, M., Chen, C., Chen, X., Zhang, M., Yuan, G., Yu, X. and Yang, Y. (2014), Protective Action of Liraglutide in Beta Cells Under Lipotoxic Stress Via PI3K/Akt/FoxO1 Pathway. J. Cell. Biochem., 115: 1166–1175. doi: 10.1002/jcb.24763
The authors declare that there is no duality of interest associated with this manuscript.
- Issue online: 15 APR 2014
- Version of Record online: 15 APR 2014
- Accepted manuscript online: 10 JAN 2014 10:34AM EST
- Manuscript Accepted: 7 JAN 2014
- Manuscript Received: 14 AUG 2013
- National Natural Science Foundation of China. Grant Numbers: 81100581, 81100582
- China International Medical Foundation (CIMF)—Novo Nordisk China β Academy Funding. Grant Number: 20110059
- China International Medical Foundation (CIMF)—Novo Nordisk China Diabetes Young Scientific. Grant Number: 2012041
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|JCB24763-sm-0001-SuppFig-S1.doc||202K||Figure S1. Protein levels of Akt, phosphorylated Akt, FoxO1, and phosphorylated FoxO1 in islets were assayed by Western blotting. C57BL/6J mice were fed with either normal chow (Control) or a high-fat diet (HF). Subcutaneous injections of liraglutide were performed to normal diet fed mice (Li) or to high-fat fed mice (Li + HF). (A) Total Akt and phosphorylated Akt (p-Akt) were detected. β-actin was used as a loading control. (B) The levels of Akt phosphorylation were expressed as p-Akt/Akt and normalized relative to non-treatment group (control group). (C) Total FoxO1 and phosphorylated FoxO1 (p-FoxO1) were detected. (D) The levels of FoxO1 phosphorylation were expressed as p-FoxO1/FoxO1 and normalized relative to non-treatment group (control group). Images are representative of three experiments with similar results. Data are presented as the means ± SEM of three independent experiments. **P < 0.01, *P < 0.05 vs. control group.|
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