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JCB24763-sm-0001-SuppFig-S1.doc202KFigure S1. Protein levels of Akt, phosphorylated Akt, FoxO1, and phosphorylated FoxO1 in islets were assayed by Western blotting. C57BL/6J mice were fed with either normal chow (Control) or a high-fat diet (HF). Subcutaneous injections of liraglutide were performed to normal diet fed mice (Li) or to high-fat fed mice (Li + HF). (A) Total Akt and phosphorylated Akt (p-Akt) were detected. β-actin was used as a loading control. (B) The levels of Akt phosphorylation were expressed as p-Akt/Akt and normalized relative to non-treatment group (control group). (C) Total FoxO1 and phosphorylated FoxO1 (p-FoxO1) were detected. (D) The levels of FoxO1 phosphorylation were expressed as p-FoxO1/FoxO1 and normalized relative to non-treatment group (control group). Images are representative of three experiments with similar results. Data are presented as the means ± SEM of three independent experiments. **P < 0.01, *P < 0.05 vs. control group.

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