Conflict of interest: The authors state that there are no conflicts of interest.
Vinculin Regulates Assembly of Talin: β3 Integrin Complexes
Article first published online: 15 APR 2014
© 2014 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry
Volume 115, Issue 6, pages 1206–1216, June 2014
How to Cite
Nanda, S. Y., Hoang, T., Patel, P. and Zhang, H. (2014), Vinculin Regulates Assembly of Talin: β3 Integrin Complexes. J. Cell. Biochem., 115: 1206–1216. doi: 10.1002/jcb.24772
- Issue published online: 15 APR 2014
- Article first published online: 15 APR 2014
- Accepted manuscript online: 20 JAN 2014 11:05PM EST
- Manuscript Accepted: 17 JAN 2014
- Manuscript Received: 10 DEC 2013
- NIH grant. Grant Number: GM41605
Additional supporting information may be found in the online version of this article at the publisher's web-site.
|jcb24772-sm-0001-SuppFig-S1.tif||739K||Fig. S1. Temperature dependent binding of talin constructs to integrin cytoplasmic tails. The pull down of EGFP-Tn constructs from lysates by β integrin cytoplasmic tails adsorbed to NTA beads was determined using the supernatant depletion assay. (A) Pull down of EGFP-Tn constructs overnight by β3CT-NTA beads increased with increasing temperatures. (B, C) Same as (A) but depicts pull down of EGFP-Talin constructs from lysates by β1D and β1 A cytoplasmic tails respectively. Each data point at different temperatures represent mean ± SEM (n = 3).|
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