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jcb24772-sm-0001-SuppFig-S1.tif739KFig. S1. Temperature dependent binding of talin constructs to integrin cytoplasmic tails. The pull down of EGFP-Tn constructs from lysates by β integrin cytoplasmic tails adsorbed to NTA beads was determined using the supernatant depletion assay. (A) Pull down of EGFP-Tn constructs overnight by β3CT-NTA beads increased with increasing temperatures. (B, C) Same as (A) but depicts pull down of EGFP-Talin constructs from lysates by β1D and β1 A cytoplasmic tails respectively. Each data point at different temperatures represent mean ± SEM (n = 3).

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