Xiao-Fei Qiang, Zheng-Wei Zhang, and Qian Liu contributed equally to this work.
miR-20a Promotes Prostate Cancer Invasion and Migration Through Targeting ABL2
Article first published online: 7 MAY 2014
© 2014 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry
Volume 115, Issue 7, pages 1269–1276, July 2014
How to Cite
Qiang, X.-F., Zhang, Z.-W., Liu, Q., Sun, N., Pan, L.-L., Shen, J., Li, T., Yun, C., Li, H. and Shi, L.-H. (2014), miR-20a Promotes Prostate Cancer Invasion and Migration Through Targeting ABL2. J. Cell. Biochem., 115: 1269–1276. doi: 10.1002/jcb.24778
- Issue published online: 7 MAY 2014
- Article first published online: 7 MAY 2014
- Accepted manuscript online: 25 JAN 2014 04:52AM EST
- Manuscript Accepted: 22 JAN 2014
- Manuscript Received: 19 NOV 2013
- Seed Foundation of Logistics University of the Chinese People's Armed Police Forces. Grant Number: FYM201112
- PROSTATE CANCER;
The aberrant expression of microRNAs (miRNAs) has been found in various types of cancer. The present study found miR-20a was significantly up-regulated in prostate cancer compared with normal prostate tissues. Patients with a higher miR-20a expression had a Gleason score of 7–10 and shorter survival time. The transwell and wound healing assays revealed that blocking expression of miR-20a by miR-20a ASO suppresses the invasion and migration of PC-3 and DU145 cells in vitro and also inhibits tumor growth in vivo. Furthermore, we identified miR-20a directly targets the ABL family non-receptor tyrosine kinases ABL2 and negatively regulates the phosphorylation of its downstream gene p190RhoGAP. Knockdown of ABL2 promoted cell invasion and migration and we identified miR-20a-induced cell invasion and migration can be rescued by ABL2. In conclusion, our findings show that miR-20a significantly contributes to the progression of prostate cancer by targeting ABL2. J. Cell. Biochem. 115: 1269–1276, 2014. © 2014 Wiley Periodicals, Inc.