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jcb24809-sm-0001-Suppl_Fig_S1.pdf540KFig. S1. TGF-β regulates fibrotic gene expression. A549 cells were cultured in serum-free medium for 24 h, after which they were treated with either vehicle control or 2 ng/ml TGF-β for 48 h. Gene expression was measured using real-time RT-PCR. Data are presented as the fold increase of expression relative to the control ± SEM.
jcb24809-sm-0002-Suppl_Fig_S2.pdf498KFig. S2. Grem1 knockdown. A549 cells were transfected for 48 h with either a nonspecific control siRNA or an siRNA targeting Gremlin 1, after which protein extracts were prepared. Immunoblot analysis was used to measure Gremlin 1 knockdown.
jcb24809-sm-0003-Suppl_Fig_S3.pdf550KFig. S3. TGF-β-induced gene expression is not regulated by BMPs. A549 cells were cultured in serum-free medium for 24 h, after which they were treated with either vehicle control, or 2 ng/ml TGF-β in the presence of absence of 50 ng/ml of the indicated BMPs. After 48 hours, gene expression was measured using real-time RT-PCR. Data are presented as the fold increase of expression relative to the control ± SEM.

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