Get access
Journal of Cellular Biochemistry

MiR-124 Inhibits Myogenic Differentiation of Mesenchymal Stem Cells Via Targeting Dlx5

Authors

  • Abdul S. Qadir,

    1. Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Korea
    Search for more papers by this author
  • Kyung Mi Woo,

    1. Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Korea
    Search for more papers by this author
  • Hyun-Mo Ryoo,

    1. Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Korea
    Search for more papers by this author
  • TacGhee Yi,

    1. Translational Research Center and Inha Research Institute for Medical Sciences, Inha University School of Medicine, Incheon, Korea
    Search for more papers by this author
  • Sun U. Song,

    1. Translational Research Center and Inha Research Institute for Medical Sciences, Inha University School of Medicine, Incheon, Korea
    Search for more papers by this author
  • Jeong-Hwa Baek

    Corresponding author
    1. Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Korea
    • Correspondence to: Jeong-Hwa Baek, Department of Molecular Genetics, School of Dentistry, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul 110-744, Korea.

      E-mail: baekjh@snu.ac.kr

    Search for more papers by this author

  • All authors state that they have no conflicts of interest.

ABSTRACT

MicroRNAs (miRNAs), including miR-1, miR-133, and miR-206, play a crucial role in muscle development by regulating muscle cell proliferation and differentiation. The aim of the present study was to define the effect of miR-124 on myogenic differentiation of mesenchymal stem cells (MSCs). The expression level of miR-124 in skeletal muscles was much lower than those in primary cultured bone marrow-derived MSCs and the bone, fat and brain tissues obtained from C57BL/6 mice. Myogenic stimuli significantly decreased the expression levels of miR-124 in mouse bone marrow-derived MSCs and C2C12 cells. Forced expression of miR-124 suppressed the expression of myogenic marker genes such as Myf5, Myod1, myogenin and myosin heavy chain and multinucleated myotube formation. Blockade of endogenous miR-124 with a hairpin inhibitor enhanced myogenic marker gene expression and myotube formation. During myogenic differentiation of MSCs and C2C12 cells, the levels of Dlx5, a known target of miR-124, were inversely regulated with those of miR-124. Furthermore, overexpression of Dlx5 increased myogenic differentiation, whereas knockdown of Dlx5 using siRNA inhibited myogenesis in C2C12 cells. These results suggest that miR-124 is a negative regulator of myogenic differentiation of MSCs and that upregulation of Dlx5 accompanied with downregulation of miR-124 by myogenic stimuli is necessary for the proper progression of myogenic differentiation. J. Cell. Biochem. 115: 1572–1581, 2014. © 2014 Wiley Periodicals, Inc.

Get access to the full text of this article

Ancillary