This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi:[10.1002/jcb.24841]
MicroRNA-365 Inhibits the Proliferation of Vascular Smooth Muscle Cells by Targeting Cyclin D1†
© 2014 Wiley Periodicals, Inc.
- Accepted manuscript online: 12 MAY 2014 06:41AM EST
- Manuscript Accepted: 8 APR 2014
- Manuscript Revised: 14 MAR 2014
- Manuscript Received: 14 NOV 2013
Additional supporting information may be found in the online version of this article at the publisher's web-site.
supporting informationFig. S1: Down-regulated miR-365 expression in vascular smooth muscle cells (VSMCs) treated with PDGF-bb and targets cyclin D1 (A) The effects of several miRNAs targeting cyclin D1 in VSMCs treated with PDGF-bb (20 ng/ml) and (B) transfection efficiency of miRs targeting cyclin D1 by realtime PCR. (C) Expression of cyclin D1 after transfection with miRs targeting cyclin D1 in VSMC were detected by western blot analysis. (*P<0.05 vs. control, #<0.05 vs. PDGF-bb-treated VSMCs).
supporting informationFig. S2: Effect of anti-miR-365 in VSMCs (A) Proliferation rate was analyzed by CCK assay after transfection with anti-miR-365 and treatment with PDGF-bb (20 ng/ml). (B,C) Expression of cyclin D1 and PCNA in VSMCs transfected with either N.C or anti-miR-365 were detected by western blot analysis (*P<0.05 vs. control).
supporting informationFig. S3: Effect of anti-miR-365 in migration of VSMCs. Analysis of cell migration as measured by a wound-healing assay. The cells were wounded with a 200 µl pipet tip and wound closure was photographed at 0 hr and 16 hr postwounding. Statistical analysis of the percent of wound closure (*P<0.05 vs. control).
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