The authors declare no potential conflict of interests.
Changes in Chromatin Structure in NIH 3T3 Cells Induced by Valproic Acid and Trichostatin A
Version of Record online: 10 SEP 2014
© 2014 Wiley Periodicals, Inc.
Journal of Cellular Biochemistry
Volume 115, Issue 11, pages 1937–1947, November 2014
How to Cite
Felisbino, M. B., Gatti, M. S. V. and Mello, M. L. S. (2014), Changes in Chromatin Structure in NIH 3T3 Cells Induced by Valproic Acid and Trichostatin A. J. Cell. Biochem., 115: 1937–1947. doi: 10.1002/jcb.24865
- Issue online: 10 SEP 2014
- Version of Record online: 10 SEP 2014
- Accepted manuscript online: 10 JUN 2014 04:33AM EST
- Manuscript Accepted: 2 JUN 2014
- Manuscript Received: 1 NOV 2013
- FAPESP. Grant Numbers: 2010/50015-6, 2009/11763-0
- CNPq. Grant Numbers: 301943/2009-5, 471303/2009-7, 132345/2010-2, 475261/2012-7
Additional supporting information may be found in the online version of this article at the publisher's web-site.
Fig. S1. Scatter diagrams of SC % versus AAR of Feulgen-stained NIH 3T3 cells. A general decrease in SC % values concomitant with an increase in AAR values is observed for nuclei in cells treated with VPA and TSA for 1 (a), 2 (b), 4 (c), or 24 h (d) in comparison with untreated controls (black dots). A: 0.05 mM VPA, dark blue square dots; (B) 1.0 mM VPA, green lozenge dots; (C) 10 ng/ml TSA, purple square dots; (D) 20 ng/ml TSA, cyanic square dots; (E) 100 ng/ml TSA, yellow square dots; n, 100.
Fig. S2. Fitted line plots for VPA- and TSA-treated NIH 3T3 cells. The relationship between nuclear areas in μm2 and Feulgen-DNA values (IOD) in arbitrary units is shown for different VPA (A–D) and TSA (E–H) concentrations. A,E—1 h; B,F—2 h; C,G—4 h; D, H—24 h; n, 100.
TABLE S1. Condensed Chromatin Area and Chromatin Textural Contrast in NIH 3T3 Cells Treated With Low VPA and TSA Concentrations
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