Cheng-Chun Lee and Wei-Yong Lin contributed equally to this work.
Association of interleukin-18 gene polymorphism with asthma in Chinese patients
Article first published online: 16 JAN 2008
© 2008 Wiley-Liss, Inc.
Journal of Clinical Laboratory Analysis
Volume 22, Issue 1, pages 39–44, 2008
How to Cite
Lee, C.-C., Lin, W.-Y., Wan, L., Tsai, Y., Tsai, C.-H., Huang, C.-M., Chen, C.-P. and Tsai, F.-J. (2008), Association of interleukin-18 gene polymorphism with asthma in Chinese patients. J. Clin. Lab. Anal., 22: 39–44. doi: 10.1002/jcla.20218
- Issue published online: 16 JAN 2008
- Article first published online: 16 JAN 2008
- Manuscript Accepted: 6 AUG 2007
- Manuscript Received: 26 SEP 2006
- China Medical University Hospital. Grant Numbers: DMR-96-119, CMU 90-185
- the National Science Council in Taiwan. Grant Number: NSC 96-2320-B-039-022
- interleukin-18 (IL-18);
- single nucleotide polymorphisms (SNPs)
Like other allergic diseases, asthma results from multiple conditions. Asthmatic beginning and severity are mediated by both environmental and genetic factors. In asthma studies, important work is realization of the genetic background and identification of genetic factors resulting in asthma development and phenomena. Here, we investigated whether interleukin (IL)-18 single nucleotide polymorphisms (SNPs) are involved in Chinese asthma patients. IL-18 (IL-18) SNP was detected by polymerase chain reaction (PCR)-based restriction analysis in 201 patients with asthma and 60 normal controls. Significant differences were found in the genotype distribution of IL-18 SNP between asthma patients and controls (P=0.000003). Allelic frequency of the IL-18 gene distinguished asthma patients from controls (P=0.000066). The results revealed a significant difference between asthma patients and normal controls in IL-18 SNP and a statistical correlation between IL-18 polymorphisms (105A/C) and asthma formation. We concluded that Chinese who carry the C/C homozygote of the IL-18-105A/C gene polymorphism in coding regions may have a higher risk of developing asthma. J. Clin. Lab. Anal. 22:39–44, 2008. © 2008 Wiley-Liss, Inc.