Grant sponsors: University Medical Center Utrecht; Julius Center for Health Sciences and Primary Care (‘Strategische Impuls’); Netherlands Laboratory for Anticancer Drug Formulation (NLADF), Amsterdam, The Netherlands.
Search for Breast Cancer Biomarkers in Fractionated Serum Samples by Protein Profiling With SELDI-TOF MS
Article first published online: 24 JAN 2012
© 2012 Wiley-Liss, Inc.
Journal of Clinical Laboratory Analysis
Volume 26, Issue 1, pages 1–9, January 2012
How to Cite
Opstal-van Winden, A. W.J., Beijnen, J. H., Loof, A., van Heerde, W. L., Vermeulen, R., Peeters, P. H.M. and van Gils, C. H. (2012), Search for Breast Cancer Biomarkers in Fractionated Serum Samples by Protein Profiling With SELDI-TOF MS. J. Clin. Lab. Anal., 26: 1–9. doi: 10.1002/jcla.20492
Additional Supporting Information may be found in the online version of this article.
- Issue published online: 24 JAN 2012
- Article first published online: 24 JAN 2012
- Manuscript Accepted: 31 AUG 2011
- Manuscript Received: 20 JUL 2011
- University Medical Center Utrecht; Julius Center for Health Sciences and Primary Care
- breast cancer;
- SELDI-TOF MS;
Many high-abundant acute phase reactants have been previously detected as potential breast cancer biomar-kers. However, they are unlikely to be specific for breast cancer. Cancer-specific biomarkers are thought to be among the lower abundant proteins.
We aimed to detect lower abundant discriminating proteins by performing serum fractionation by strong anion exchange chromatography preceding protein profiling with SELDI-TOF MS. In a pilot study, we tested the different fractions resulting from fractionation, on several array types. Fraction 3 on IMAC30 and Fraction 6 on Q10 yielded the most discriminative proteins and were used for serum protein profiling of 73 incident breast cancer cases and 73 matched controls.
Eight peaks showed statistically significantly different intensities between cases and controls (P⧁0.05), and had less than 10% chance to be a false-positive finding. Seven of these were tentatively identified as apolipoprotein C-II (m/z 8,909), oxidized apolipoprotein C-II (m/z 8,925), apolipoprotein C-III (m/z 8,746), fragment of coagulation factor XIIIa (m/z 3,959), heterodimer of apolipoprotein A-I and apolipoprotein A-II (m/z 45,435), hemoglobin B-chain (m/z 15,915), and post-translational modified hemoglobin (m/z 15,346).
By extensive serum fractionation, we detected many more proteins than in previous studies without fractionation. However, discriminating proteins were still high abundant. Results indicate that either lower abundant proteins are less distinctive, or more rigorous fractionation and selective protein depletion, or a more sensitive assay, are needed to detect lower abundant discriminative proteins.