Dynamic visualization of expressed gene networks
Article first published online: 19 MAY 2003
Copyright © 2003 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 196, Issue 3, pages 419–429, September 2003
How to Cite
Remy, I. and Michnick, S. W. (2003), Dynamic visualization of expressed gene networks. J. Cell. Physiol., 196: 419–429. doi: 10.1002/jcp.10328
- Issue published online: 9 JUL 2003
- Article first published online: 19 MAY 2003
- Manuscript Accepted: 5 MAR 2003
- Manuscript Received: 26 FEB 2003
Cellular biochemical machineries, what we call pathways, consist of dynamically assembling and disassembling macromolecular complexes. While our models for the organization of biochemical machines are derived largely from in vitro experiments, do they reflect their organization in living cells? We have developed a general experimental strategy that addresses this question by allowing the quantitative probing of molecular interactions in intact living cells. The experimental strategy is based on protein fragment complementation assays (PCA), a method whereby protein interactions are coupled to refolding of enzymes from cognate fragments where reconstitution of enzyme activity acts as the detector of a protein interaction. A biochemical machine or pathway is defined by grouping interacting proteins into those that are perturbed in the same way by common factors (hormones, metabolites, enzyme inhibitors, etc). In this review, we describe how we go from descriptive to quantitative representations of biochemical networks at an individual to whole genome level and how our approach will lead ultimately to better descriptions of the biochemical machineries that underlie living processes. J. Cell. Physiol. 196: 419–429, 2003. © 2003 Wiley-Liss, Inc.