Research sponsored by the U. S. Atomic Energy Commission under contract with the Union Carbide Corporation. The data were presented in part to the University of Tennessee by V. K. Jenkins as partial fulfillment of the requirement for the Ph.D. degree while he was supported by U.S.P.H.S. Fellowship 5-F1-GM-30,763-02.
Differences between exogenous and endogenous hemopoietic spleen colonies†
Article first published online: 4 FEB 2005
Copyright © 1969 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 73, Issue 2, pages 141–148, April 1969
How to Cite
Jenkins, V. K., Upton, A. C. and Odell, T. T. (1969), Differences between exogenous and endogenous hemopoietic spleen colonies. J. Cell. Physiol., 73: 141–148. doi: 10.1002/jcp.1040730208
- Issue published online: 4 FEB 2005
- Article first published online: 4 FEB 2005
- Manuscript Accepted: 28 FEB 1969
- Manuscript Received: 1 NOV 1968
Histologic examination of the spleens in RFM/Un mice killed 6 to 9 days after 350 to 800 R whole-body x-irradiation revealed hemopoietic colonies, the numbers of which decreased exponentially with increasing radiation dose. In such animals, myelocytic colonies were the predominant type on the sixth to the eighth day. However, they decreased in number with time, being fewer than erythropoietic colonies by the ninth day after irradiation. In C57BL mice, erythropoietic colonies were relatively more numerous, markedly predominating on both the eighth and the thirteenth days.
RFM/Un mice injected with nonirradiated syngeneic bone marrow cells within 24 hours after 750 R developed colonies, predominantly of erythropoietic and undifferentiated types, the numbers of which were proportional to the numbers of marrow cells injected. The number of colonies formed from exogenous marrow cells increased slightly between the sixth and ninth days after inoculation, possibly because of a greater likelihood of counting them due to an increase in their size.