This investigation was supported by Public Health Service Training Grant CA-5013, from the National Cancer Institute.
Development of the mouse hematopoietic system. II. Estimation of spleen and liver “stem” cell number†
Article first published online: 4 FEB 2005
Copyright © 1969 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 74, Issue 1, pages 51–55, August 1969
How to Cite
Barker, J. E., Keenan, M. A. and Raphals, L. (1969), Development of the mouse hematopoietic system. II. Estimation of spleen and liver “stem” cell number. J. Cell. Physiol., 74: 51–55. doi: 10.1002/jcp.1040740107
- Issue published online: 4 FEB 2005
- Article first published online: 4 FEB 2005
- Manuscript Accepted: 7 MAY 1969
- Manuscript Received: 30 JAN 1969
Colony-forming cells (CFU), which have the general properties of hemopoietic “stem” cells, appear to be augmented in the mouse fetal liver from 12–18 days gestation and then decrease in the newborn. This finding suggests that few, if any, hemopoietic “stem” cells remain in the adult liver, an organ which appears to be unable to function erythropoietically, even at times of severe crises. In the spleen, and active adult as well as embryonic hematopoietic organ, the total number of CFU increases from 18 days gestation until at least 7 days after birth.
Spleen and liver CFU augmentation seems to occur in cojunction with an analogous expansion of non-hematopoietic cells. The data suggests, in fact, that while there is an increase in the total number of liver CFU, there is also a dilution of liver CFU in the total cell population at successively later gestational ages.