Supported by grants from the American Cancer society (E-498) and the National Cancer Institute, National Institutes of Health (I ROI CA 11305-DI).
Human bone marrow colony growth in agar-gel†
Article first published online: 4 FEB 2005
Copyright © 1970 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 76, Issue 1, pages 77–84, August 1970
How to Cite
Pike, B. L. and Robinson, W. A. (1970), Human bone marrow colony growth in agar-gel. J. Cell. Physiol., 76: 77–84. doi: 10.1002/jcp.1040760111
- Issue published online: 4 FEB 2005
- Article first published online: 4 FEB 2005
- Manuscript Accepted: 6 MAR 1970
- Manuscript Received: 22 DEC 1969
A technique for growing human bone marrow cell colonies in agar-gel medium is described. “Feeder layers” containing 1 × 106 normal human peripheral white blood cells are used as the stimulus for colony growth. Human bone marrow aspirates are collected in heparinized syringes and plated as 2 × 105 cells on “feeder layers.” Normal human bone marrow yields 32–102 colonies per 2 × 105 cells plated. Colonies are almost exclusively granulocytic. Growth rate of colonies is slower than with mouse bone marrow but colonies reach a comparable size (500–1500 cells) at days 12–16.