Formerly trainees in the Department of Cell Biology, Albert Einstein College of Medicine where some initial experiments were performed.
Contact inhibition, polyribosomes, and cell surface membranes in cultured mammalian cells†
Article first published online: 4 FEB 2005
Copyright © 1974 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 84, Issue 3, pages 349–363, December 1974
How to Cite
Levine, E. M., Jeng, D.-Y. and Chang, Y. (1974), Contact inhibition, polyribosomes, and cell surface membranes in cultured mammalian cells. J. Cell. Physiol., 84: 349–363. doi: 10.1002/jcp.1040840304
This research was supported by U. S. Public Health Service Grant GM20306 and National Science Foundation Grant GB-35590. During the initial stages of this work, E. M. L. was the recipient of a Career Development Award (K3-AI-8532) from the National Institutes of Health. Subsequent support was provided by grants (RK-05540 and CA-10315) from the Division of Research Resources and The National Cancer Institute, National Institutes of Health. The expert technical assistance of Miss Suzanne Arelt, Mrs. Barbara Becker, and Miss Barbara Dooner is gratefully acknowledged.
- Issue published online: 4 FEB 2005
- Article first published online: 4 FEB 2005
- Manuscript Accepted: 20 MAY 1974
- Manuscript Received: 11 FEB 1974
An “overlay” method for rapidly and synchronously inducing contact inhibition in normal cultured cells has been developed. Using this method, disaggregation of cytoplasmic polyribosomes has been observed to occur within a matter of hours after overlay, followed by a decrease in cellular ribosomal RNA. Polysome disaggregation was influenced by the extent of cell-cell interaction and was inhibited by pretreatment of overlay cells with cycloheximide. Treatment of underlay cells with cytosine arabinoside also induced polysome disaggregation, but only after an appreciable lag as compared to that observed in overlaid cultures. Disaggregation could be induced by this method in cultured cells derived from normal tissue but not in cells derived from cancerous tissue. Polysome synthesis in growing “normal” cells (as measured by incorporation of tracer uridine into RNA) was markedly decreased when a cell surface membrane preparation was added to cultures.