Hemopoietic colony forming cells in regenerating mouse liver

Authors

  • Esther F. Hays,

    1. Laboratory of Nuclear Medicine and Radiation Biology, Department of Medicine, University of California, Center for Health Sciences, Los Angeles, California, 90024
    2. Department of Surgery, the Childrens Hospital of Los Angeles, University of Southern California School of Medicine, Los Angeles, California, 90054
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  • Frank C. Firkin,

    1. Laboratory of Nuclear Medicine and Radiation Biology, Department of Medicine, University of California, Center for Health Sciences, Los Angeles, California, 90024
    2. Department of Surgery, the Childrens Hospital of Los Angeles, University of Southern California School of Medicine, Los Angeles, California, 90054
    Current affiliation:
    1. Department of Medicine, St. Vincents Hospital, Fitzroy 3065, Victoria, Australia
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    • Frank C. Firkin was a recipient of the Bushell's traveling fellowship of the Royal Australian College of Physicians.

  • Yoshiko Koga,

    1. Laboratory of Nuclear Medicine and Radiation Biology, Department of Medicine, University of California, Center for Health Sciences, Los Angeles, California, 90024
    2. Department of Surgery, the Childrens Hospital of Los Angeles, University of Southern California School of Medicine, Los Angeles, California, 90054
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  • Daniel M. Hays

    1. Laboratory of Nuclear Medicine and Radiation Biology, Department of Medicine, University of California, Center for Health Sciences, Los Angeles, California, 90024
    2. Department of Surgery, the Childrens Hospital of Los Angeles, University of Southern California School of Medicine, Los Angeles, California, 90054
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  • Supported by Contract AT (04–1) GEN-12 between the Atomic Energy Commission and the University of California and by Public Health Service Grants CA 13666 from the National Cancer Institute, and AM 08879 from the National Institute of Arthritis, Metabolism and Digestive Diseases.

Abstract

Cells from regenerating mouse liver removed 2–25 days post 68% hepatic resection have been assayed for in vitro colony forming capacity in soft agar (CFU-C), proliferative capacity in liquid culture, and in vivo spleen colony forming capacity (CFU-S).

These studies demonstrated low concentrations of CFU-C and CFU-S in normal and sham-operated liver, with an appreciable increase of both in regenerating liver, reaching maximum values in tissue removed 5–7 days post hepatic resection. Colony formation in agar by regenerating liver cells occurred in the absence of exogenous colony stimulating factor. Separation of liver cells on the basis of adherent properties prior to culture indicated concentration of CFU-C in the nonadherent fraction, while cells producing colony stimulating factor were concentrated in the adherent fraction. Foci of actively dividing cells of the macrophage and granulocyte series arose in liquid culture from preparations of sham operated and regenerating liver, although total cell formation was greater with regenerating liver. A small proportion of the colonies formed in agar from regenerating liver consisted of cords of epithelioid cells, which resembled hepatocytes and differed from the macrophages or granulocytes found in the majority of colonies, raising the possibility that regenerating hepatocytes form colonies in agar culture.

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