The influence of cell population density and serum on the (Na+K)-ATPase of 3T3 cells

Authors

  • Brian McCaldin,

    Corresponding author
    1. Department of Physiology and Pharmacology, University of St. Andrews, St. Andrews, Fife KY16 9TS, Scotland
    • Department of Physiology, National University of Malaysia, P.O Box 12418, Jalan Raja Muda, Kuala Lumpur, Malaysia
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  • Effendi M. Tenang

    1. Department of Physiology and Pharmacology, University of St. Andrews, St. Andrews, Fife KY16 9TS, Scotland
    Current affiliation:
    1. Hof Reit 37 1/2 D8019 Moosach, W. Germany
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Abstract

The (Na+K)-ATPase of 3T3 cells has been measured as a function of culture density and the type of serum in which the cells were grown. When 3T3 cells were grown in medium containing 10% newborn calf serum their (Na+K)-ATPase activity increased as the culture density increased and the cells became quiescent at densities of about 17.5 × 104 cells/cm2. When these cells are subcultured into medium containing 10% foetal bovine serum the activity of the enzyme decreases as the culture density increases and the cells attain final densities of about 5 × 104 cells/cm2.

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