Reinitiation of cellular DNA synthesis in BrdU-selected nondividing senescent WI-38 cells by simian virus 40 infection
Article first published online: 4 FEB 2005
Copyright © 1985 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 125, Issue 1, pages 122–126, October 1985
How to Cite
Gorman, S. D. and Cristofalo, V. J. (1985), Reinitiation of cellular DNA synthesis in BrdU-selected nondividing senescent WI-38 cells by simian virus 40 infection. J. Cell. Physiol., 125: 122–126. doi: 10.1002/jcp.1041250116
- Issue published online: 4 FEB 2005
- Article first published online: 4 FEB 2005
- Manuscript Accepted: 17 MAY 1985
- Manuscript Received: 3 DEC 1984
Bromodeoxyuridine-selected nondividing senescent WI-38 cells were stimulated to synthesize DNA, as evidenced by incorporation of [3H]thymidine into nuclei of senescent cells, after infection with simian virus 40 (SV40). Cellular DNA synthesis was confirmed by DNA-DNA hybridization experiments and the use of temperature-sensitive A gene mutants. The DNA synthesis was, at least in part, semiconservative, as microdensitometry of Feulgen-stained nuclei revealed increased DNA content in a large fraction of the cells in the infected population. Thus, senescent cells retain the capacity to replicate their DNA, despite their intrinsic inability to initiate DNA synthesis.