Long-term maintenance of hepatocyte functional activity in co-culture: Requirements for sinusoidal endothelial cells and dexamethasone

Authors

  • Odette Morin,

    Corresponding author
    1. Department of Medicine, Laval University, and Laval University Cancer Research Center, Hǒtel-Dieu de Québec, Québec, Canada G1R 2J6
    • Department of Medicine, Laval University, and Laval University Cancer Research Center, Hǒtel-Dieu de Québec, Québec, Canada G1R 2J6
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  • Claire Normand

    1. Department of Medicine, Laval University, and Laval University Cancer Research Center, Hǒtel-Dieu de Québec, Québec, Canada G1R 2J6
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Abstract

Sinusoidal cells isolated from adult rat liver have been established in primary culture and in cell line. The presence of factor VIII R:Ag and peroxidatic/phagocytosis activities were the criteria used to distinguish in freshly isolated cells the endothelial cells from the Kupffer cells and suggested the endothelial origin of the cell line. Using a co-culture system, the effect of sinusoidal liver cells on hèpatocyte functional activity was characterized. A plateau in which the state of differentiation was stabilized could be generated for co-cultured hepatocytes isolated from adult rat and a disappearance of the initial expression of α1-fetoprotein (AFP) and the increase and/or maintenance of albumin secretion were measured with co-cultured hepatocytes isolated from suckling rat. The presence of dexamethasone was required for such beneficial effect. The hepatocyte-stabilizing activity was also produced by a pulmonary endothelial cell line.

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