Novel regulatory actions of 1α,25-dihydroxyvitamin D3 on the metabolism of polyphosphoinositides in murine epidermal keratinocytes
Article first published online: 4 FEB 2005
Copyright © 1987 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 132, Issue 1, pages 131–136, July 1987
How to Cite
Tang, W., Ziboh, V. A., Isseroff, R. R. and Martinez, D. (1987), Novel regulatory actions of 1α,25-dihydroxyvitamin D3 on the metabolism of polyphosphoinositides in murine epidermal keratinocytes. J. Cell. Physiol., 132: 131–136. doi: 10.1002/jcp.1041320118
- Issue published online: 4 FEB 2005
- Article first published online: 4 FEB 2005
- Manuscript Accepted: 22 DEC 1986
- Manuscript Received: 25 AUG 1986
The in vitro incubation of murine keratinocytes in the presence of 1α,25-dihydroxyvitamin D3 enhanced the rapid hydrolysis of the prelabeled keratinocyte polyphosphoinositides (polyPtdIns) when compared to untreated cells. The rapid hydrolysis of the polyPtdIns and the release of the inositol phosphosphates (particularly InsP3 and InsP2) precede the onset of differentiation of these cells. These data therefore suggest that 1α,25-didydroxyvitamin D3 functions in vitro to initiate the rapid generation of InsP3 from cellular polyPtdIns; this in turn may mobilize intracellular Ca2+, thus providing the signal which program the murine keratinocytes from a proliferating mode into a differentiating mode.