Effect of albumin on 14C-α-Methyl-d-Glucopyranoside uptake in primary cultured renal proximal tubule cells: Involvement of PLC, MAPK, and NF-κB
Article first published online: 18 JUN 2004
Copyright © 2005 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 202, Issue 1, pages 246–254, January 2005
How to Cite
Han, H. J., Oh, Y. J. and Lee, Y. J. (2005), Effect of albumin on 14C-α-Methyl-d-Glucopyranoside uptake in primary cultured renal proximal tubule cells: Involvement of PLC, MAPK, and NF-κB. J. Cell. Physiol., 202: 246–254. doi: 10.1002/jcp.20108
- Issue published online: 28 OCT 2004
- Article first published online: 18 JUN 2004
- Manuscript Accepted: 25 MAR 2004
- Manuscript Received: 26 DEC 2003
- Ministry of Science and Technology, Republic of Korea (Stem Cell Research Center of 21st Century Frontier Research Program). Grant Number: SC14032
A growing body of evidence implicates albumin has an important regulatory function in renal proximal tubule cells (PTCs). In present study, the effect of bovine serum albumin (BSA) on 14C-α-methyl-d-glucopyranoside (α-MG) uptake and its related signal molecules were examined in the primary cultured rabbit renal PTCs. BSA significantly increased uptake of α-MG, a distinctive proximal tubule marker, as well as expression level of Na+/glucose cotransporters (SGLT1 and SGLT2) proteins. The BSA-induced increase of α-MG uptake was completely blocked by actinomycin D and cycloheximide. Neomycin or U 73122 (PLC inhibitors), BAPTA/AM or TMB-8 (intracellular Ca2+ mobilization inhibitors) completely abolished BSA-induced increase of α-MG uptake. BSA significantly increased IPs accumulation, but did not affect Ca2+ uptake. Effect of BSA on α-MG uptake was blocked by PD 98059, but did not SB 203580. BSA increased phosphorylation of p44/42 mitogen activated protein kinase (MAPK) in a time-dependent manner. NAC or catalase (antioxidants) significantly blocked BSA-induced increase of H2O2 formation and α-MG uptake. BSA activated NF-κB translocation into nucleus. PDTC, SN50, and TLCK (NF-κB inhibitors) also completely blocked BSA-induced increase of α-MG uptake, NF-κB p65 and phospho IκB-α activation. In conclusion, BSA stimulates α-MG uptake and its action is partially correlated with PLC, MAPK, or NF-κB signal molecules in primary cultured renal PTCs. © 2005 Wiley-Liss, Inc.