Polyoma virus middle-T-transformed PECAM-1 deficient mouse brain endothelial cells proliferate rapidly in culture and form hemangiomas in mice
Article first published online: 22 JUN 2004
Copyright © 2005 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 202, Issue 1, pages 230–239, January 2005
How to Cite
Rothermel, T. A., Engelhardt, B. and Sheibani, N. (2005), Polyoma virus middle-T-transformed PECAM-1 deficient mouse brain endothelial cells proliferate rapidly in culture and form hemangiomas in mice. J. Cell. Physiol., 202: 230–239. doi: 10.1002/jcp.20114
- Issue published online: 28 OCT 2004
- Article first published online: 22 JUN 2004
- Manuscript Accepted: 19 MAR 2004
- Manuscript Received: 18 FEB 2004
- National Institutes of Health. Grant Numbers: AR45599, EY13700
Wild-type mouse brain endothelial (bEND) cells transformed with the polyoma virus middle-T proliferate rapidly in culture and form hemangiomas in mice. These cells express high levels of platelet/endothelial cell adhesion molecule-1 (PECAM-1), a molecule shown to be important during hemangioma formation. In this study, we have examined the ability of polyoma virus middle-T-transformed mouse bEND cells prepared from PECAM-1−/− mice to proliferate in culture and form hemangiomas in mice. We show that these cells express a number of endothelial cell markers and share a similar morphology with PECAM-1+/+ bEND cells. PECAM-1−/− bEND cells exhibit a limited ability to form tubes in Matrigel and rapidly form hemangioma when injected into nude mice, very similar to PECAM-1+/+ bEND cells. These cells, however, have increased proliferation, slower migration, altered endothelial cell adhesion molecule expression, and are less adherent when compared to PECAM-1+/+ bEND cells. Therefore, lack of PECAM-1 expression impacts polyoma middle-T-transformed endothelial cell proliferative, adhesive, and migratory properties without impacting their ability to rapidly form hemangiomas in mice or poorly organize to capillary-like structures in Matrigel. © 2005 Wiley-Liss, Inc.