Cysteine-protease involved in male chromatin remodeling after fertilization co-localizes with α-tubulin at mitosis
Article first published online: 5 AUG 2004
Copyright © 2004 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 202, Issue 2, pages 602–607, February 2005
How to Cite
Concha, C., Morin, V., Bustos, P., Genevière, A. M., Heck, M. M.S., Puchi, M. and Imschenetzky, M. (2005), Cysteine-protease involved in male chromatin remodeling after fertilization co-localizes with α-tubulin at mitosis. J. Cell. Physiol., 202: 602–607. doi: 10.1002/jcp.20157
- Issue published online: 23 NOV 2004
- Article first published online: 5 AUG 2004
- Manuscript Accepted: 20 MAY 2004
- Manuscript Received: 16 APR 2004
- FONDECYT. Grant Number: 011073
- DIUC. Grant Number: 200.031.088-10
- International visiting exchange: ECOS France/CONICYT Chile and Royal Society U.K./CONICYT Chile
We postulated an essential role for a cysteine-protease in sea urchins sperm histones degradation which follows fertilization. We now report the purification of this enzyme, the determination of its N-terminal amino acid sequence and the localization of the protein with antibodies generated against this amino-terminal peptide. The immunofluorescence data confirmed the presence of this enzyme in the nucleus of unfertilized eggs. After fertilization labeling is observed both in female and male pronuclei suggesting a rapid recruitment of the enzyme to the male pronuclei. Interestingly, we have found that this cysteine-protease persists in the nucleus of the zygotes during S phase of the cell cycle and co-localizes with α-tubulin that organizes the mitotic spindle during the initial embryonic cell division. © 2004 Wiley-Liss, Inc.