Gas6 induces proliferation in prostate carcinoma cell lines expressing the Axl receptor
Article first published online: 16 DEC 2004
Copyright © 2004 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 204, Issue 1, pages 36–44, July 2005
How to Cite
Sainaghi, P. P., Castello, L., Bergamasco, L., Galletti, M., Bellosta, P. and Avanzi, G. C. (2005), Gas6 induces proliferation in prostate carcinoma cell lines expressing the Axl receptor. J. Cell. Physiol., 204: 36–44. doi: 10.1002/jcp.20265
- Issue published online: 25 APR 2005
- Article first published online: 16 DEC 2004
- Manuscript Accepted: 8 OCT 2004
- Manuscript Received: 21 SEP 2004
- Università del Piemonte Orientale
Axl is a tyrosine kinase receptor and although it is expressed in malignancy such as leukemia, colon cancer, melanoma, endometrial, prostate and thyroid cancers, its role has not been completely elucidated yet and appears to be complex. The ligand of Axl, Gas6, is a 75 KDa multimodular protein with an N-terminal gamma-carboxy-glutamic acid that is essential for binding. Gas6 has a mitogenic effect on several normal cell lines. The receptor Axl is expressed in primary prostate carcinoma and in prostate cancer cell lines as such as PC-3 and DU 145. We demonstrated a mitogenic activity determined by Gas6/Axl interaction in these undifferentiated metastatic human prostatic cancer cell lines. This effect is proportional to Axl expression, not due to inhibition of apoptosis, and induces AKT and MAPK phosphorylation. However, only MEK phosphorylation seems to be essential for growth signaling. Our results suggest that Axl overexpression and activation by Gas6 could be involved in progression of prostate neoplastic disease. © 2004 Wiley-Liss, Inc.