Signature of B-CLL with different prognosis by Shrunken centroids of surface antigen expression profiling
Article first published online: 16 DEC 2004
Copyright © 2004 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 204, Issue 1, pages 113–123, July 2005
How to Cite
Zucchetto, A., Sonego, P., Degan, M., Bomben, R., Bo, M. D., Russo, S., Attadia, V., Rupolo, M., Buccisano, F., Principe, M. I. D., Poeta, G. D., Pucillo, C., Colombatti, A., Campanini, R. and Gattei, V. (2005), Signature of B-CLL with different prognosis by Shrunken centroids of surface antigen expression profiling. J. Cell. Physiol., 204: 113–123. doi: 10.1002/jcp.20269
- Issue published online: 25 APR 2005
- Article first published online: 16 DEC 2004
- Manuscript Accepted: 26 OCT 2004
- Manuscript Received: 24 AUG 2004
- Ministero della Salute (Ricerca Finalizzata I.R.C.C.S.), Rome, Italy
- Ministero della Salute (“Alleanza Contro il Cancro”), Rome, Italy
With the aim of identifying the immunophenotypic profile of B-cell chronic lymphocytic leukemia (B-CLL) subsets with different prognosis, we investigated by flow cytometry the expression of 36 surface antigens in 123 cases, all with survivals. By analyzing results with unsupervised (hierarchical and K-means clustering) algorithms, three distinct immunophenotypic groups (I, II, and III) were identified, group I (51/123) with longer survivals, as compared to the group II (36/123) and III (36/123). The immunophenotypic signatures of these groups, as determined by applying the nearest Shrunken centroids method as class predictor, were characterized by the coordinated and differential expression of 12 surface markers, that is, group I: above-average expression of CD62L, CD54, CD49c, and CD25, below-average expression of CD38; group II: above-average expression of CD38, CD49d, CD29, and CD49e; and group III: below-average expression of the above markers, overexpression of CD23, CD20, SmIg, and CD79b. As opposed to groups II–III, group I B-CLLs lacked expression of ZAP-70 and activation-induced cytidine deaminase in the majority of cases, while more frequently had mutated IgVH genes and IgVH mutations consistent with antigen-driven selection. Our findings contribute to improve the immunophenotypical identification of disease subsets with different prognosis and suggest a set of surface antigens to be employed as prognosticators in routine diagnostic/prognostic procedures. © 2004 Wiley-Liss, Inc.