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This article includes Supplementary Material available from the authors upon request or via the Internet at http://www.interscience.wiley.com/jpages/0021-9541/suppmat .

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jws-jcp.20636.fig1.tif5862KFig. S1: FSC and SSC analysis of peripheral blood. FACS analysis of peripheral blood by FSC (cell size) and SSC (granularity). Blue dots are cells positive for each CD antigen: CD4 (helper T lymphocytes), CD8 (cytotoxic T lymphocytes), CD11b (both granulocytes and monocytes/macrophages), CD14 (monocytes/macrophages), CD15 (granulocytes), and CD19 (B lymphocytes). Granulocytes, monocytes/macrophages, and lymphocytes are located predominantly in the upper, middle, and lower populations, respectively.
jws-jcp.20636.fig2.tif4574KFig. S2: Multicolor FACS analysis of peripheral blood. Blue dots are cells positive for CD4 (helper T lymphocytes), CD8 (cytotoxic T lymphocytes), CD14 (monocytes/macrophages), and CD15 (granulocytes). All blood-derived cells were CD45 + . Granulocytes and monocytes/macrophages were CD31 + , while lymphocytes were CD31 - .
jws-jcp.20636.fig3.tif22622KFig. S3: Multicolor FACS analysis of freshly isolated HUVEC, cultured HUVEC, and cultured dermal fibroblasts. Freshly isolated HUVEC (a), cultured HUVEC (EGM-2; passage 2) (b), and cultured dermal fibroblasts (DMEM+10%FBS, passage 4) (c) were analyzed for CD31, CD34, CD45, and one of following markers: CD90, CD105, or CD146. (a) All freshly isolated cells from human umbilical cord vein were plotted in the graph at far left, showing contamination by blood-derived CD45 +cells (red dots). Only adipose-derived CD45 -cells (blue dots) were shown in the six graphs at right. Most freshly isolated HUVEC were CD31 + CD34 + CD45 - CD105 + CD146 + , while CD90 expression varied. (b) Most cultured HUVEC (passage 2) were CD31 + CD34 - CD45 - CD90 - CD105 + CD146 + . Small percentages of CD31 + CD34 + CD45 - CD90 - CD105 + CD146 +and CD31 + CD34 - CD45 - CD90 + CD105 + CD146 +cells were also detected. Red and blue dots are CD34 +and CD34 -cells, respectively. (c) Most of the cultured dermal fibroblasts were CD31 - CD34 - CD45 - CD90 + CD105 - CD146 - , and a small percentage was CD31 - CD34 + CD45 - CD90 + CD105 - CD146 - . Red and blue dots are CD34 +and CD34 -cells, respectively, gated in the graph at far left.

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