Kotaro Yoshimura and Tomokuni Shigeura contributed equally to the study.
Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates†
Article first published online: 23 MAR 2006
Copyright © 2006 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 208, Issue 1, pages 64–76, July 2006
How to Cite
Yoshimura, K., Shigeura, T., Matsumoto, D., Sato, T., Takaki, Y., Aiba-Kojima, E., Sato, K., Inoue, K., Nagase, T., Koshima, I. and Gonda, K. (2006), Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J. Cell. Physiol., 208: 64–76. doi: 10.1002/jcp.20636
- Issue published online: 21 APR 2006
- Article first published online: 23 MAR 2006
- Manuscript Accepted: 31 JAN 2006
- Manuscript Received: 12 NOV 2005
- Japanese Ministry of Education, Culture, Sports, Science, and Technology (MEXT). Grant Number: B2-16390507
This article includes Supplementary Material available from the authors upon request or via the Internet at http://www.interscience.wiley.com/jpages/0021-9541/suppmat .
|jws-jcp.20636.fig1.tif||5862K||Fig. S1: FSC and SSC analysis of peripheral blood. FACS analysis of peripheral blood by FSC (cell size) and SSC (granularity). Blue dots are cells positive for each CD antigen: CD4 (helper T lymphocytes), CD8 (cytotoxic T lymphocytes), CD11b (both granulocytes and monocytes/macrophages), CD14 (monocytes/macrophages), CD15 (granulocytes), and CD19 (B lymphocytes). Granulocytes, monocytes/macrophages, and lymphocytes are located predominantly in the upper, middle, and lower populations, respectively.|
|jws-jcp.20636.fig2.tif||4574K||Fig. S2: Multicolor FACS analysis of peripheral blood. Blue dots are cells positive for CD4 (helper T lymphocytes), CD8 (cytotoxic T lymphocytes), CD14 (monocytes/macrophages), and CD15 (granulocytes). All blood-derived cells were CD45 + . Granulocytes and monocytes/macrophages were CD31 + , while lymphocytes were CD31 - .|
|jws-jcp.20636.fig3.tif||22622K||Fig. S3: Multicolor FACS analysis of freshly isolated HUVEC, cultured HUVEC, and cultured dermal fibroblasts. Freshly isolated HUVEC (a), cultured HUVEC (EGM-2; passage 2) (b), and cultured dermal fibroblasts (DMEM+10%FBS, passage 4) (c) were analyzed for CD31, CD34, CD45, and one of following markers: CD90, CD105, or CD146. (a) All freshly isolated cells from human umbilical cord vein were plotted in the graph at far left, showing contamination by blood-derived CD45 +cells (red dots). Only adipose-derived CD45 -cells (blue dots) were shown in the six graphs at right. Most freshly isolated HUVEC were CD31 + CD34 + CD45 - CD105 + CD146 + , while CD90 expression varied. (b) Most cultured HUVEC (passage 2) were CD31 + CD34 - CD45 - CD90 - CD105 + CD146 + . Small percentages of CD31 + CD34 + CD45 - CD90 - CD105 + CD146 +and CD31 + CD34 - CD45 - CD90 + CD105 + CD146 +cells were also detected. Red and blue dots are CD34 +and CD34 -cells, respectively. (c) Most of the cultured dermal fibroblasts were CD31 - CD34 - CD45 - CD90 + CD105 - CD146 - , and a small percentage was CD31 - CD34 + CD45 - CD90 + CD105 - CD146 - . Red and blue dots are CD34 +and CD34 -cells, respectively, gated in the graph at far left.|
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