Increased mobilization of c-kit+ Sca-1+ Lin− (KSL) cells and colony-forming units in spleen (CFU-S) following de novo formation of a stem cell niche depends on dynamic, but not stable, membranous ossification
Article first published online: 30 MAR 2006
Copyright © 2006 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 208, Issue 1, pages 188–194, July 2006
How to Cite
Nagayoshi, K., Ohkawa, H., Yorozu, K., Higuchi, M., Higashi, S., Kubota, N., Fukui, H., Imai, N., Gojo, S., Hata, J.-i., Kobayashi, Y. and Umezawa, A. (2006), Increased mobilization of c-kit+ Sca-1+ Lin− (KSL) cells and colony-forming units in spleen (CFU-S) following de novo formation of a stem cell niche depends on dynamic, but not stable, membranous ossification. J. Cell. Physiol., 208: 188–194. doi: 10.1002/jcp.20652
- Issue published online: 21 APR 2006
- Article first published online: 30 MAR 2006
- Manuscript Accepted: 13 FEB 2006
- Manuscript Received: 26 JUL 2005
- Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan. Grant Numbers: 14081208, 13470053, 14657051
- Health and Labour Sciences Research Grants. Grant Numbers: H-14-trans-003, KH71064
- Pharmaceuticals and Medical Devices Agency. Grant Number: 02-2
This article includes Supplementary Material available from the authors upon request or via the Internet at http://www.interscience.wiley.com/jpages/0021-9541/suppmat .
|jws-jcp.20652.fig1.tif||3251K||Supplementary Figure 1S---RT-PCR analysis of the expression of cytokine genes in KUSA-A1 cells and KUSA-O cells---Total RNA was isolated from the cultured cells and, after reverse transcription, a multiplex polymerase chain reaction with primers for the 23 genes was performed. After 40 cycles of amplification, the samples were electrophoresed and stained with ethidium bromide. The gene expression pattern was reproducibly observed in two separate experiments. A. RT-PCR analysis of the house-keeping genes, i.e., the succinate dehydrogenase (SDHA), hypoxanthine guanine phosphoribosyl transferase 1 (HPRT), and |*beta*|-2-microglobulin (B2M) genes. B. RT-PCR analysis (MM1001) of the colony-stimulating factor-1 (CSF-1), thrombopoietin (THPO), colony-stimulating factor-3 (CSF-3: granulocyte-CSF), angiotensinogen (ANGT2, arrowhead), granulocyte-macrophage CSF (CSF2), and erythropoietin (EPO) genes. C. RT-PCR analysis (MM1003) of the c-kit ligand (KITL), leptin (LEP), lymphotoxin A (LTA), lymphotoxin B (LTB), and interleukin-19 (IL19) genes. D. RT-PCR analysis (MM1005) of the interleukin-5 (IL5), interleukin-10 (IL10), interleukin-16 (IL16), interleukin-12B (IL12B), interleukin-17B (IL17B), interleukin-6 (IL6), and interleukin-4 (IL4) genes. E. RT-PCR analysis (MM1501) of the angiogenesis genes, i.e., the protease activated receptor 1 (F2R, arrowhead), FMS-like tyrosine kinase 1 (FLT1), FMS-like tyrosine kinase 4 (FLT4), Tie2, and angiopoietin1 (ANGPT1, arrowhead) genes. F. Ethidium bromide-stained 28S and 18S rRNA. P: Positive size markers, lane 1: KUSA-O cells, lane 2: KUSA-A1 cells.|
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