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jws-jcp.20652.fig1.tif3251KSupplementary Figure 1S---RT-PCR analysis of the expression of cytokine genes in KUSA-A1 cells and KUSA-O cells---Total RNA was isolated from the cultured cells and, after reverse transcription, a multiplex polymerase chain reaction with primers for the 23 genes was performed. After 40 cycles of amplification, the samples were electrophoresed and stained with ethidium bromide. The gene expression pattern was reproducibly observed in two separate experiments. A. RT-PCR analysis of the house-keeping genes, i.e., the succinate dehydrogenase (SDHA), hypoxanthine guanine phosphoribosyl transferase 1 (HPRT), and |*beta*|-2-microglobulin (B2M) genes. B. RT-PCR analysis (MM1001) of the colony-stimulating factor-1 (CSF-1), thrombopoietin (THPO), colony-stimulating factor-3 (CSF-3: granulocyte-CSF), angiotensinogen (ANGT2, arrowhead), granulocyte-macrophage CSF (CSF2), and erythropoietin (EPO) genes. C. RT-PCR analysis (MM1003) of the c-kit ligand (KITL), leptin (LEP), lymphotoxin A (LTA), lymphotoxin B (LTB), and interleukin-19 (IL19) genes. D. RT-PCR analysis (MM1005) of the interleukin-5 (IL5), interleukin-10 (IL10), interleukin-16 (IL16), interleukin-12B (IL12B), interleukin-17B (IL17B), interleukin-6 (IL6), and interleukin-4 (IL4) genes. E. RT-PCR analysis (MM1501) of the angiogenesis genes, i.e., the protease activated receptor 1 (F2R, arrowhead), FMS-like tyrosine kinase 1 (FLT1), FMS-like tyrosine kinase 4 (FLT4), Tie2, and angiopoietin1 (ANGPT1, arrowhead) genes. F. Ethidium bromide-stained 28S and 18S rRNA. P: Positive size markers, lane 1: KUSA-O cells, lane 2: KUSA-A1 cells.

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