An unusual extended DNA loop attachment region is located in the human dystrophin gene
Article first published online: 1 AUG 2006
Copyright © 2006 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 209, Issue 2, pages 515–521, November 2006
How to Cite
Iarovaia, O. V., Borounova, V., Vassetzky, Y. S. and Razin, S. V. (2006), An unusual extended DNA loop attachment region is located in the human dystrophin gene. J. Cell. Physiol., 209: 515–521. doi: 10.1002/jcp.20759
- Issue published online: 29 AUG 2006
- Article first published online: 1 AUG 2006
- Manuscript Accepted: 16 JUN 2006
- Manuscript Received: 15 JUN 2006
- The Association Française des Myopathies
- The Russian Academy of Sciences. Grant Number: 06-04-48358a
We have found and characterized an unusual extended area of DNA association with the nuclear matrix in the human dystrophin gene. This extended DNA loop anchorage region (LAR) has been mapped and characterized using a variety of biochemical and microscopy techniques. It spans approximately 200 kbp at chromosomal locations 950–1,150 Kb downstream to the beginning of the first exon of the dystrophin gene Dp427m and covers a part of the intron 43, exon 44, and most of intron 44. The extended LAR harbors the major recombination hot spot of the dystrophin gene and a replication origin. We propose a model where DNA topoisomerase II-mediated cleavage at the nuclear matrix may enhance recombination events within this extended LAR. J. Cell. Physiol. 209: 515–521, 2006. © 2006 Wiley-Liss, Inc.