Mario Mikula and Verena Proell contributed equally to this work.
Activated hepatic stellate cells induce tumor progression of neoplastic hepatocytes in a TGF-β dependent fashion†
Article first published online: 1 AUG 2006
Copyright © 2006 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 209, Issue 2, pages 560–567, November 2006
How to Cite
Mikula, M., Proell, V., Fischer, A.N.M. and Mikulits, W. (2006), Activated hepatic stellate cells induce tumor progression of neoplastic hepatocytes in a TGF-β dependent fashion. J. Cell. Physiol., 209: 560–567. doi: 10.1002/jcp.20772
- Issue published online: 29 AUG 2006
- Article first published online: 1 AUG 2006
- Manuscript Accepted: 26 JUN 2006
- Manuscript Received: 8 MAR 2006
- Austrian Science Fund (FWF). Grant Number: SFB F28
- Jubiläumsfonds der Oesterreichischen Nationalbank. Grant Number: OENB 10171
- Herzfelder'sche Familienstiftung
This article includes Supplementary Material available from the authors upon request or via the Internet at http://www.interscience.wiley.com/jpages/0021-9541/suppmat .
|jws-jcp.20772.fig1.tif||1173K||Supplementary figure 1. TGF-β1 production of MIM-1-4 hepatocytes (white bar), M1-4HSC cells (grey bar) and myofibroblastoid M-HT cells (black bar). The amount of latent TGF-β1 secretion into the medium was determined by ELISA.|
|jws-jcp.20772.fig2.tif||1338K||Supplementary figure 2. Repression of Smad-dependent transactivation by exogenous expression of Smad7. A: Smad7 expression as detected by Immunoblotting. B: Hepatocytes were transiently transfected with a luciferase reporter construct responsive to Smad3-mediated transcriptional activation and treated with TGF-β1 at the indicated concentrations. White bars, MIM-1-4; light gray bars, MIM-1-4-S7; MIM-R (dark gray bars); MIM-RS7 (black bars).|
|jws-jcp.20772.fig3.tif||1263K||Supplementary figure 3. Proliferation kinetics of epithelial MIM hepatocytes in the presence and absence of TGF-β1. A starting population of 1 x 105 cells was cultured on collagen-coated tissue plates to monitor cumulative cell numbers. A: MIM-1-4 and MIM-R hepatocytes and those expressing exogenous Smad7 (MIM-1-4-S7 and MIM-RS7) in the absence of TGF-β1. B: MIM-1-4, MIM-R, MIM-1-4-S7 and MIM-RS7 treated with TGF-β1 (1 ng/ml).|
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