Original Article

Multipotential human adipose-derived stromal stem cells exhibit a perivascular phenotype in vitro and in vivo

  1. A.C.W. Zannettino1,
  2. S. Paton2,
  3. A. Arthur2,
  4. F. Khor2,
  5. S. Itescu3,
  6. J.M. Gimble4,
  7. S. Gronthos2,*

Article first published online: 24 JUL 2007

DOI: 10.1002/jcp.21210

Issue

Journal of Cellular Physiology

Journal of Cellular Physiology

Volume 214, Issue 2, pages 413–421, February 2008

Additional Information

How to Cite

Zannettino, A.C.W., Paton, S., Arthur, A., Khor, F., Itescu, S., Gimble, J.M. and Gronthos, S. (2008), Multipotential human adipose-derived stromal stem cells exhibit a perivascular phenotype in vitro and in vivo. J. Cell. Physiol., 214: 413–421. doi: 10.1002/jcp.21210

Author Information

  1. 1

    Myeloma Research Program, Bone and Cancer Laboratories, Division of Haematology, Institute of Medical and Veterinary Science, Hanson Institute and University of Adelaide, Adelaide, Australia

  2. 2

    Mesenchymal Stem Cell Group, Bone and Cancer Laboratories, Division of Haematology, Institute of Medical and Veterinary Science, Hanson Institute and University of Adelaide, Adelaide, Australia

  3. 3

    Department of Medicine, University of Melbourne, Melbourne, Australia and Department of Transplantation Immunology, Columbia University, New York—Presbyterian Hospital, New York

  4. 4

    Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, Louisiana

*Division of Haematology, Institute of Medical and Veterinary Science, Frome Road, Adelaide 5000, Australia.

Publication History

  1. Issue published online: 21 NOV 2007
  2. Article first published online: 24 JUL 2007
  3. Manuscript Accepted: 8 JUN 2007
  4. Manuscript Received: 20 APR 2007

Funded by

  • Australian National Health and Research Council Project. Grant Number: 242804

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Abstract

Mesenchymal stem-like cells identified in different tissues reside in a perivascular niche. In the present study, we investigated the putative niche of adipose-derived stromal/stem cells (ASCs) using markers, associated with mesenchymal and perivascular cells, including STRO-1, CD146, and 3G5. Immunofluorescence staining of human adipose tissue sections, revealed that STRO-1 and 3G5 co-localized with CD146 to the perivascular regions of blood vessels. FACS was used to determine the capacity of the CD146, 3G5, and STRO-1 specific monoclonal antibodies to isolate clonogenic ASCs from disassociated human adipose tissue. Clonogenic fibroblastic colonies (CFU-F) were found to be enriched in those cell fractions selected with either STRO-1, CD146, or 3G5. Flow cytometric analysis revealed that cultured ASCs exhibited similar phenotypic profiles in relation to their expression of cell surface markers associated with stromal cells (CD44, CD90, CD105, CD106, CD146, CD166, STRO-1, alkaline phosphatase), endothelial cells (CD31, CD105, CD106, CD146, CD166), haematopoietic cells (CD14, CD31, CD45), and perivascular cells (3G5, STRO-1, CD146). The immunoselected ASCs populations maintained their characteristic multipotential properties as shown by their capacity to form Alizarin Red positive mineralized deposits, Oil Red O positive lipid droplets, and Alcian Blue positive proteoglycan-rich matrix in vitro. Furthermore, ASCs cultures established from either STRO-1, 3G5, or CD146 selected cell populations, were all capable of forming ectopic bone when transplanted subcutaneously into NOD/SCID mice. The findings presented here, describe a multipotential stem cell population within adult human adipose tissue, which appear to be intimately associated with perivascular cells surrounding the blood vessels. J. Cell. Physiol. 214: 413–421, 2008. © 2007 Wiley-Liss, Inc.

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