Butyrate and vitamin D3 induce transcriptional attenuation at the cyclin D1 locus in colonic carcinoma cells
Article first published online: 25 NOV 2008
Copyright © 2008 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 218, Issue 3, pages 638–642, March 2009
How to Cite
Maier, S., Daroqui, M. C., Scherer, S., Roepcke, S., Velcich, A., Shenoy, S. M., Singer, R. H. and Augenlicht, L. H. (2009), Butyrate and vitamin D3 induce transcriptional attenuation at the cyclin D1 locus in colonic carcinoma cells. J. Cell. Physiol., 218: 638–642. doi: 10.1002/jcp.21642
- Issue published online: 18 DEC 2008
- Article first published online: 25 NOV 2008
- Manuscript Accepted: 15 OCT 2008
- Manuscript Received: 15 JUL 2008
- National Cancer Institute. Grant Numbers: U54 CA100926, R21 CA123473, R33 CA83208, PO13330
In stimulating maturation of colonic carcinoma cells, the short chain fatty acid butyrate, and 1α,25-dihydroxyvitamin D3, were shown to attenuate transcription of the cyclin D1 gene, giving rise to truncated transcripts of this locus. Moreover, a sequence which is highly conserved in the human, mouse, rat, and dog genome was found in the 4 kb long intron 3 of the human cyclin D1 gene, and is capable of forming a hairpin structure similar to that of microRNA precursors. The expression of this sequence is also decreased by the attenuation. Thus, the transcriptional attenuation at the cyclin D1 locus not only down-regulates the expression of this key gene in mucosal cell maturation and tumorigenesis, but may also abrogate the generation of a molecule that encompasses this conserved sequence in cyclin D1 intron 3. J. Cell. Physiol. 218: 638–642, 2009. © 2008 Wiley-Liss, Inc.