Expression of proinflammatory cytokines by human mesenchymal stem cells in response to cyclic tensile strain
Article first published online: 16 DEC 2008
Copyright © 2008 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 219, Issue 1, pages 77–83, April 2009
How to Cite
Sumanasinghe, R. D., Pfeiler, T. W., Monteiro-Riviere, N. A. and Loboa, E. G. (2009), Expression of proinflammatory cytokines by human mesenchymal stem cells in response to cyclic tensile strain. J. Cell. Physiol., 219: 77–83. doi: 10.1002/jcp.21653
- Issue published online: 20 JAN 2009
- Article first published online: 16 DEC 2008
- Manuscript Accepted: 30 OCT 2008
- Manuscript Received: 23 JAN 2007
- Ralph E. Powe Junior Faculty Enhancement Award
- North Carolina Biotechnology Center Institutional Development
Mesenchymal stem cells produce proinflammatory cytokines during their normal growth. Direct or indirect regulation of bone resorption by these cytokines has been reported. However, the effects of osteogenic conditions—chemical and/or mechanical—utilized during in vitro bone tissue engineering on expression of cytokines by hMSCs have not been studied. In this study, we investigated the effects of cyclic tensile strain, culture medium (with and without dexamethasone), and culture duration on the expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-8 (IL-8) by bone marrow derived human mesenchymal stem cells (hMSCs). Human MSCs seeded in three-dimensional Type I collagen matrices were subjected to 0%, 10%, and 12% uniaxial cyclic tensile strains at 1 Hz for 4 h/day for 7 and 14 days in complete growth or dexamethasone-containing osteogenic medium. Viability of hMSCs was maintained irrespective of strain level and media conditions. Expression of either TNF-α or IL-1β was not observed in hMSCs under any of the conditions investigated in this study. Expression of IL-6 was dependent on culture medium. An increase in IL-6 expression was caused by both 10% and 12% strain levels. Both 10% and 12% strain levels caused an increase in IL-8 production by hMSCs that was dependent on the presence of dexamethasone. IL-6 and IL-8 expressions by hMSCs were induced by cyclic tensile strain and osteogenic differentiating media, indicating that IL-6 and IL-8 may be functioning as autocrine signals during osteogenic differentiation of hMSCs. J. Cell. Physiol. 219: 77–83, 2009. © 2008 Wiley-Liss, Inc.