All contributing authors declared no conflicts of interest to disclose, whether financial or of any other nature.
Der p2 activates airway smooth muscle cells in a TLR2/MyD88-dependent manner to induce an inflammatory response†
Article first published online: 26 MAR 2009
Copyright © 2009 Wiley-Liss, Inc.
Journal of Cellular Physiology
Volume 220, Issue 2, pages 311–318, August 2009
How to Cite
Chiou, Y.-L. and Lin, C.-Y. (2009), Der p2 activates airway smooth muscle cells in a TLR2/MyD88-dependent manner to induce an inflammatory response. J. Cell. Physiol., 220: 311–318. doi: 10.1002/jcp.21764
- Issue published online: 26 MAY 2009
- Article first published online: 26 MAR 2009
- Manuscript Accepted: 17 FEB 2009
- Manuscript Received: 16 JAN 2009
Der p2 is a major allergen from Dermatophagoides pteronyssinus, the main species of house dust mite and a major inducer of asthma, inducing harmful respiratory inflammatory responses by activating cells in the respiratory tract, leading to an unstable status. We hypothesize that Der p2 may induce local inflammatory responses by directly affecting airway smooth muscle (ASM) cells. In this study, we demonstrated that Der p2 raised nuclear factor-kappa B (NF-κB) and extracellular signal-regulated kinase (ERK) 1/2 activation and induced a high level of proinflammatory cytokines expression in primary cultured ASM cells. Der p2 activated the MyD88 signaling pathway through toll-like receptor (TLR) 2, not through TLR4. Notably, Der p2 stimulated ASM cells to increase phosphorylation of ERK1/2 and expression of c-Fos, which were also important in the T helper type 2 (Th2) immune response. These results suggest that Der p2 induces asthma through the MyD88 signaling pathway in respiratory tissue. J. Cell. Physiol. 220: 311–318, 2009. © 2009 Wiley-Liss, Inc.