Original Research Article
Proteomics in Ménière disease†
Article first published online: 24 OCT 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Cellular Physiology
Volume 227, Issue 1, pages 308–312, January 2012
How to Cite
Chiarella, G., Saccomanno, M., Scumaci, D., Gaspari, M., Faniello, M. C., Quaresima, B., Di Domenico, M., Ricciardi, C., Petrolo, C., Cassandro, C., Costanzo, F. S., Cuda, G. and Cassandro, E. (2012), Proteomics in Ménière disease. J. Cell. Physiol., 227: 308–312. doi: 10.1002/jcp.22737
Giuseppe Chiarella and Milena Saccomanno contributed equally to this work.
- Issue published online: 24 OCT 2011
- Article first published online: 24 OCT 2011
- Accepted manuscript online: 24 MAR 2011 09:56AM EST
- Manuscript Accepted: 14 MAR 2011
- Manuscript Received: 26 JAN 2011
Ménière's disease (MD) is a disorder of the inner ear characterized by an insidious onset and aspecific symptoms, such as dizziness, vertigo, tinnitus, and hearing loss, that may become very debilitating. The presence of endolymphatic hydrops is a common feature in MD patients, but the pathophysiology is still largely unknown. In this study, we have used a proteomics-driven approach to identify potential biomarkers of MD. To this end, plasma was obtained from whole blood of 16 individuals previously diagnosed as suffering from MD and compared to plasma from healthy donors. A depletion of the highly abundant proteins (i.e., albumin, IgG, transferrin, etc.) was performed in order to enhance the chance of detection of the less represented ones, therefore reducing the noise-background. Two-dimensional gel electrophoresis, followed by in-gel tryptic digestion of the selected spots and LC-MS/MS analysis, allowed us to identify a set of proteins whose expression appears to be differentially modulated in patients versus controls. In particular: complement factor H and B, fibrinogen alpha and gamma chains, beta-actin and pigment epithelium derived factor are over expressed; on the other hand, the levels of beta-2 glycoprotein-1, vitamin D binding protein and apolipoprotein-1 are significantly decreased in the plasma of MD-affected individuals. Even though preliminary and not necessarily linked directly to the molecular pathogenesis of the disease, our original findings suggest that a molecular signature, represented by the plasma protein profile previously described, might represent a potentially powerful, innovative and not invasive tool for early diagnosis and clinical management of MD patients. J. Cell. Physiol. 227: 308–312, 2012. © 2011 Wiley Periodicals, Inc.