Get access

A multipotent clonal human periodontal ligament cell line with neural crest cell phenotypes promotes neurocytic differentiation, migration, and survival

Authors

  • Atsushi Tomokiyo,

    1. Faculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan
    Search for more papers by this author
  • Hidefumi Maeda,

    Corresponding author
    1. Faculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan
    • Faculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, 3-1-1 Maidashi, Fukuoka 812-8582, Japan.
    Search for more papers by this author
  • Shinsuke Fujii,

    1. Faculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan
    Search for more papers by this author
  • Satoshi Monnouchi,

    1. Faculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan
    Search for more papers by this author
  • Naohisa Wada,

    1. Faculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan
    Search for more papers by this author
  • Kiyomi Kono,

    1. Faculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan
    Search for more papers by this author
  • Naohide Yamamoto,

    1. Faculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan
    Search for more papers by this author
  • Katsuaki Koori,

    1. Faculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan
    Search for more papers by this author
  • Yoko Teramatsu,

    1. Faculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan
    Search for more papers by this author
  • Akifumi Akamine

    1. Faculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan
    Search for more papers by this author

Abstract

Repair of injured peripheral nerve is thought to play important roles in tissue homeostasis and regeneration. Recent experiments have demonstrated enhanced functional recovery of damaged neurons by some types of somatic stem cells. It remains unclear, however, if periodontal ligament (PDL) stem cells possess such functions. We recently developed a multipotent clonal human PDL cell line, termed cell line 1-17. Here, we investigated the effects of this cell line on neurocytic differentiation, migration, and survival. This cell line expressed the neural crest cell marker genes Slug, SOX10, Nestin, p75NTR, and CD49d and mesenchymal stem cell-related markers CD13, CD29, CD44, CD71, CD90, CD105, and CD166. Rat adrenal pheochromocytoma cells (PC12 cells) underwent neurocytic differentiation when co-cultured with cell line 1-17 or in conditioned medium from cell line 1-17 (1-17CM). ELISA analysis revealed that 1-17CM contained approximately 50 pg/ml nerve growth factor (NGF). Cell line 1-17-induced migration of PC12 cells, which was inhibited by a neutralizing antibody against NGF. Furthermore, 1-17CM exerted antiapoptotic effects on differentiated PC12 cells as evidenced by inhibition of neurite retraction, reduction in annexin V and caspase-3/7 staining, and induction of Bcl-2 and Bcl-xL mRNA expression. Thus, cell line 1-17 promoted neurocytic differentiation, migration, and survival through secretion of NGF and possibly synergistic factors. PDL stem cells may play a role in peripheral nerve reinnervation during PDL regeneration. J. Cell. Physiol. 227: 2040–2050, 2012. © 2011 Wiley Periodicals, Inc.

Get access to the full text of this article

Ancillary