Heparin-integrin interaction in endothelial cells: Downstream signaling and heparan sulfate expression

Authors

  • Valquíria P. Medeiros,

    1. Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil
    Search for more papers by this author
  • Edgar J. Paredes-Gamero,

    1. Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil
    Search for more papers by this author
  • Hugo P. Monteiro,

    1. Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil
    Search for more papers by this author
  • Hugo A.O. Rocha,

    1. Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil
    Search for more papers by this author
  • Edvaldo S. Trindade,

    1. Departamento de Biologia Celular, Universidade Federal do Paraná, Rua Francisco H. dos Santos, Curitiba, PR, Brazil
    Search for more papers by this author
  • Helena B. Nader

    Corresponding author
    1. Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil
    • Universidade Federal de São Paulo,Escola Paulista de Medicina, Departamento de Bioquímica, Rua Três de Maio 100, 04044-020, São Paulo, SP, Brazil.
    Search for more papers by this author

Abstract

Endothelial cells (ECs) are a source of physiologically important molecules that are synthesized and released to the blood and/or to the subendothelial extracellular matrix such as a heparan sulfate proteoglycan (HSPG) with antithrombotic properties. Previously, we have shown that heparin stimulates the synthesis and modifies the sulfation pattern of this HSPG. Here the molecular mechanisms involved in the up-regulation of HSPG synthesis by heparin in endothelial cells were decoded. The cells were stimulated with heparin and the expression of HSPG and intracellular pathways were evaluated by a combination of methods involving confocal microscopy, flow cytometry, Western blotting analyses, and [35S]-sulfate metabolically labeling of the cells. We observed that the up-regulation of HSPG synthesis evoked by heparin is dependent on the interaction of heparin with integrin since RGD peptide abolishes the effect. The activation of integrin leads to tyrosine-phosphorylation of focal adhesion-associated proteins such as FAK, Src, and paxillin. In addition, heparin induces ERK1/2 phosphorylation and inhibitors of Ras and MEK decreased heparin-dependent HSPG synthesis. Moreover, heparin also induced intracellular Ca2+ release, PLCγ1 (phospholipase Cγ1) and CaMKII (calcium calmodulin kinase II) activation, as well as an increase in nitric oxide (NO) production. Finally, an intracellular Ca2+ chelator, Ca2+ signaling inhibitors, and an endothelial NO synthase inhibitor were all able to abolish the effect in heparan sulfate synthesis. In conclusion, the heparin-induced up-regulation of HSPG expression is associated with the phosphorylation of focal adhesion proteins and Ras/Raf/MEK/ERK MAP and Ca2+/NO pathways. J. Cell. Physiol. 227: 2740–2749, 2012. © 2011 Wiley Periodicals, Inc.

Ancillary