Conflicts of interest: None.
Original Research Article
Augmented LPS responsiveness in type 1 diabetes-derived osteoclasts†
Article first published online: 25 OCT 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Cellular Physiology
Volume 228, Issue 2, pages 349–361, February 2013
How to Cite
Catalfamo, D. L., Calderon, N. L., Harden, S. W., Sorenson, H. L., Neiva, K. G. and Wallet, S. M. (2013), Augmented LPS responsiveness in type 1 diabetes-derived osteoclasts. J. Cell. Physiol., 228: 349–361. doi: 10.1002/jcp.24138
- Issue published online: 25 OCT 2012
- Article first published online: 25 OCT 2012
- Accepted manuscript online: 20 JUN 2012 07:02AM EST
- Manuscript Accepted: 7 JUN 2012
- Manuscript Received: 13 MAY 2012
- American Diabetes Association. Grant Numbers: 1-08-JF-37, 7-11-CD-17
- NIH/NIDCR. Grant Number: 5T32DE007200
- NIH/NIDCR. Grant Number: 1F31DE021618-01A1
Bone abnormalities are frequent co-morbidities of type 1 diabetes (T1D) and are principally mediated by osteoblasts and osteoclasts which in turn are regulated by immunologic mediators. While decreased skeletal health in T1D involves alterations in osteoblast maturation and function, the effect of altered immune function on osteoclasts in T1D-associated bone and joint pathologies is less understood. Here T1D-associated osteoclast-specific differentiation and function in the presence and absence of inflammatory mediators was characterized utilizing bone marrow-derived osteoclasts (BM-OCs) isolated from non-obese diabetic (NOD) mice, a model for spontaneous autoimmune diabetes with pathology similar to individuals with T1D. Differentiation and osteoclast-mediated bone resorption were evaluated along with cathepsin K, MMP-9, and immune soluble mediator expression. The effect of lipopolysaccharide (LPS), a pro-inflammatory cytokine cocktail, and NOD-derived conditioned supernatants on BM-OC function was also determined. Although NOD BM-OCs cultures contained smaller osteoclasts, they resorbed more bone concomitant with increased cathepsin K, MMP-9, and pro-osteoclastogenic mediator expression. NOD BM-OCs also displayed an inhibition of LPS-induced deactivation that was not a result of soluble mediators produced by NOD BM-OCs, although a pro-inflammatory milieu did enhance NOD BM-OCs bone resorption. Together these data indicate that osteoclasts from a T1D mouse model hyper-respond to RANK-L resulting in excessive bone degradation via enhanced cathepsin K and MMP-9 secretion concomitant with an increased expression of pro-osteoclastic soluble mediators. Our data also suggest that inhibition of LPS-induced deactivation in NOD-derived BM-OC cultures is most likely due to NOD osteoclast responsiveness rather than LPS-induced expression of soluble mediators. J. Cell. Physiol. 228: 349–361, 2013. © 2012 Wiley Periodicals, Inc.