Original Research Article
Regulation of intracellular pH by glycosaminoglycans
Version of Record online: 20 JUN 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Cellular Physiology
Volume 228, Issue 10, pages 2071–2075, October 2013
How to Cite
Kathagen, N. and Prehm, P. (2013), Regulation of intracellular pH by glycosaminoglycans. J. Cell. Physiol., 228: 2071–2075. doi: 10.1002/jcp.24376
- Issue online: 20 JUN 2013
- Version of Record online: 20 JUN 2013
- Accepted manuscript online: 5 APR 2013 12:14AM EST
- Manuscript Accepted: 25 MAR 2013
- Manuscript Received: 13 FEB 2013
- Deutsche Forschungsgemeinschaft. Grant Number: SFB492
The intracellular pH is regulated by a delicate balance of ion distribution across the plasma membrane and the physico-chemical properties of intra- and extracellular components. We analyzed the effects of glycosaminoglycans on the intracellular pH of fibroblasts by using the fluorescent pH indicator BCECF-AM. Addition of hyaluronan, hyaluronan oligosaccharides, chondroitin sulfate, or heparin to the culture medium of fibroblasts caused intracellular acidification from pH 7.2 to 6.7 in a concentration dependent manner. High molecular weight hyaluronan acidified more than hyaluronan oligosaccharides at the same concentrations. Hyaluronidase treatment or inhibition of hyaluronan export with xanthohumol led to intracellular alkalization. These observations indicated that extracellular glycosaminoglycans participate in intracellular pH regulation. The mechanism was explained by Donnan effects and molecular crowding. J. Cell. Physiol. 228: 2071–2075, 2013. © 2013 Wiley Periodicals, Inc.