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jcp24377-sm-0001-SupFig-S1.tif731KFig. S1. Purification of KLF4 protein. pKLF4-myc-purio coding either the full-length KLF4 protein or an empty vector was overexpressed in 293FT cell. Forty-eight hours after transfection, cells were harvested and lysed. Protein lysates were immunoprecipitated with Myc-agarose beads. The purity and concentration of the eluted protein were determined by coomassie brilliant blue staining (upper panel) and by Western blot (lower panel).

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