Original Research Article
Stretching Impacts Inflammation Resolution in Connective Tissue
Version of Record online: 10 DEC 2015
© 2015 Wiley Periodicals, Inc.
Journal of Cellular Physiology
Volume 231, Issue 7, pages 1621–1627, July 2016
How to Cite
Berrueta, L., Muskaj, I., Olenich, S., Butler, T., Badger, G. J., Colas, R. A., Spite, M., Serhan, C. N. and Langevin, H. M. (2016), Stretching Impacts Inflammation Resolution in Connective Tissue. J. Cell. Physiol., 231: 1621–1627. doi: 10.1002/jcp.25263
- Issue online: 16 MAR 2016
- Version of Record online: 10 DEC 2015
- Accepted manuscript online: 20 NOV 2015 01:53PM EST
- Manuscript Accepted: 18 NOV 2015
- Manuscript Received: 16 NOV 2015
- Bernard Osher Foundation
- National Institutes of Health. Grant Number: PO1GM095467
Acute inflammation is accompanied from its outset by the release of specialized pro-resolving mediators (SPMs), including resolvins, that orchestrate the resolution of local inflammation. We showed earlier that, in rats with subcutaneous inflammation of the back induced by carrageenan, stretching for 10 min twice daily reduced inflammation and improved pain, 2 weeks after carrageenan injection. In this study, we hypothesized that stretching of connective tissue activates local pro-resolving mechanisms within the tissue in the acute phase of inflammation. In rats injected with carrageenan and randomized to stretch versus no stretch for 48 h, stretching reduced inflammatory lesion thickness and neutrophil count, and increased resolvin (RvD1) concentrations within lesions. Furthermore, subcutaneous resolvin injection mimicked the effect of stretching. In ex vivo experiments, stretching of connective tissue reduced the migration of neutrophils and increased tissue RvD1 concentration. These results demonstrate a direct mechanical impact of stretching on inflammation-regulation mechanisms within connective tissue. J. Cell. Physiol. 231: 1621–1627, 2016. © 2015 Wiley Periodicals, Inc.