Kinetics of glucoamylase hydrolysis of corn starch

Authors

  • Jin-Peng Wang,

    1. State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tianjin University, Tianjin 300072, PR China
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  • Ai-Wu Zeng,

    Corresponding author
    1. State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tianjin University, Tianjin 300072, PR China
    • State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tianjin University, Tianjin 300072, PR China
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  • Zhen Liu,

    1. State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tianjin University, Tianjin 300072, PR China
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  • Xi-Gang Yuan

    1. State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tianjin University, Tianjin 300072, PR China
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Abstract

Corn starch was hydrolysed by glucoamylase (EC 3.2.1.3, 1,4-a-D-glucan glucohydrolase) in a chemostat; 106 g L−1 reducing sugar was produced at 40 °C after 46 h, whereas only 42 g L−1 was produced at 30 °C. When corn starch concentration was increased from 64.6 g L−1 to 161.5 g L−1, the amount of reducing sugar produced increased with the initial substrate concentration. The studies show that there is a product concentration (p0) above which product inhibition becomes apparent. A new model for starch hydrolysis considering the product inhibition point is presented. Copyright © 2006 Society of Chemical Industry

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