A simple method for biocatalyst immobilization using PVA-based hydrogel particles

Authors

  • Pedro Fernandes,

    Corresponding author
    1. IBB, Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, 1049-001 Lisboa, Portugal
    • IBB, Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, 1049-001 Lisboa, Portugal.
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  • Marco P.C. Marques,

    1. IBB, Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, 1049-001 Lisboa, Portugal
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  • Filipe Carvalho,

    1. IBB, Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, 1049-001 Lisboa, Portugal
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  • Joaquim M.S. Cabral

    1. IBB, Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, 1049-001 Lisboa, Portugal
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Abstract

BACKGROUND: The aim of this study was to evaluate the feasibility of enzyme immobilization in PVA particles through extrusion of LentiKat®Liquid in polyethylene glycol. Inulinase, with invertase activity for sucrose hydrolysis, was used as model system.

RESULTS: Inulinase was effectively immobilized in PVA particles. The pH optimum of the enzyme activity was broadened for lower pH values. Mechanical instability of the PVA under prolonged incubation above 55 °C was observed. A 1.8-fold increase in the apparent KM (Michaelis constant) suggests diffusion limitations as a result of immobilization. The immobilized biocatalyst exhibited considerable operational stability, since a decrease of roughly 10% in the product yield after 24 h biotransformation runs was observed in trials performed at 50 °C, following 20 repeated, consecutive batches.

CONCLUSION: The results obtained highlight the potential of PVA-based particles obtained through extrusion into PEG for the production of suitable biocatalysts for application in large-scale processes. Copyright © 2008 Society of Chemical Industry

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