Research Article

Optimization of inulinase production by solid-state fermentation in a packed-bed bioreactor

  1. Marcio A. Mazutti1,2,*,
  2. Giovani Zabot2,
  3. Gabriela Boni2,
  4. Aline Skovronski2,
  5. Débora de Oliveira2,
  6. Marco Di Luccio2,
  7. Maria Isabel Rodrigues1,
  8. Helen Treichel2,*,
  9. Francisco Maugeri1

Article first published online: 1 OCT 2009

DOI: 10.1002/jctb.2273

Issue

Journal of Chemical Technology and Biotechnology

Journal of Chemical Technology and Biotechnology

Volume 85, Issue 1, pages 109–114, January 2010

Additional Information

How to Cite

Mazutti, M. A., Zabot, G., Boni, G., Skovronski, A., de Oliveira, D., Di Luccio, M., Rodrigues, M. I., Treichel, H. and Maugeri, F. (2010), Optimization of inulinase production by solid-state fermentation in a packed-bed bioreactor. J. Chem. Technol. Biotechnol., 85: 109–114. doi: 10.1002/jctb.2273

Author Information

  1. 1

    Department of Food Engineering, UNICAMP, P.O Box 6121, CEP 13083-862, Campinas—SP, Brazil

  2. 2

    Department of Food Engineering, URI—Campus de Erechim, P.O. Box 743, CEP 99700-000, Erechim—RS, Brazil

*Department of Food Engineering, URI—Campus de Erechim, P.O. Box 743, CEP 99700-000, Erechim—RS, Brazil.

Publication History

  1. Issue published online: 1 DEC 2009
  2. Article first published online: 1 OCT 2009
  3. Manuscript Accepted: 15 AUG 2009
  4. Manuscript Revised: 13 AUG 2009
  5. Manuscript Received: 9 JUL 2009

Funded by

  • CAPES. Grant Number: PROCAD/CAPES 0337/05-6
  • FAPERGS
  • CNPQ
  • URI—Campus Erechim

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Keywords:

  • solid-state;
  • packed-bed bioreactor;
  • optimization;
  • agroindustrial residues

Abstract

BACKGROUND: This work is focused on inulinase production by solid-sate fermentation (SSF) using sugarcane bagasse, corn steep liquor (CSL), pre-treated cane molasses, and soybean bran as substrates in a 3-kg (dry basis) packed-bed bioreactor. SSF was carried out by the yeast Kluyveromyces marxianus NRRL Y-7571 and response surface methodology was used to optimize the temperature, air flow rate and initial mass of cells.

RESULTS: The optimum inulinase activity (436.7 ± 36.3 U g−1 dry substrate) was obtained at 24 h at an inlet air temperature of 30 °C, air flow rate 2.2 m3 h−1 and 22 g of cells for fermentation. Inulinase productivity at these conditions was 18.2 U gds−1 h−1. Kinetic evaluation at the optimized conditions showed that the maximum inulinase production was verified at 24 h of fermentation. The carbon dioxide and the metabolic heat generation are directly associated with the consumption of total reducing sugars present in the medium.

CONCLUSION: The high productivity achieved in this work shows the technical viability of inulinase production by SSF in a packed-bed bioreactor. Copyright © 2009 Society of Chemical Industry

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